Department of Virology II, National Institute of Infectious Diseases, Tokyo, Japan.
Gastroenterology. 2013 Jan;144(1):56-58.e7. doi: 10.1053/j.gastro.2012.09.017. Epub 2012 Sep 19.
Hepatitis C virus (HCV) genotype 3a is widespread worldwide, but no replication system exists for its study. We describe a subgenomic replicon system for HCV genotype 3a. We determined the consensus sequence of an HCV genome isolated from a patient, and constructed a subgenomic replicon using this clone. The replicon was transfected into HuH-7 cells and RNA replication was confirmed. We identified cell culture-adaptive mutations that increased colony formation multiple-fold. We have therefore established a genotype 3a replicon system that can be used to study this HCV genotype.
丙型肝炎病毒（HCV）基因型 3a 在全球广泛传播，但目前尚无针对该基因型的研究复制系统。我们描述了一种丙型肝炎病毒基因型 3a 的亚基因组复制子系统。我们确定了从患者中分离出的 HCV 基因组的共识序列，并使用该克隆构建了一个亚基因组复制子。将该复制子转染入 HuH-7 细胞，证实了 RNA 复制。我们鉴定了可使集落形成增加数倍的细胞培养适应性突变。因此，我们建立了一种基因型 3a 复制子系统，可用于研究该 HCV 基因型。
