Saha Supradip, Walia Suresh, Kundu Aditi, Sharma Khushbu, Paul Ranjit Kumar
Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110 012, India.
Division of Agricultural Chemicals, Indian Agricultural Research Institute, New Delhi 110 012, India.
Food Chem. 2015 Jun 15;177:369-75. doi: 10.1016/j.foodchem.2015.01.039. Epub 2015 Jan 13.
Accelerated solvent extraction (ASE) is applied for the extraction of carotenoids from orange carrot and the extraction parameters were optimized. Two carotenoids, lutein and β-carotene, are selected as the validation process. Hildebrand solubility parameters and dielectric constant of solvents were taken into consideration in selecting solvent mixture. The effects of various experimental parameters, such as temperature, static time, drying agent etc., on the ASE extraction efficiency are investigated systematically. Interactions among the variables were also studied. Furthermore, two carotenoids were analyzed and characterized by LC-ESI MS. The study concluded that Hildebrand solubility parameter approach may be applicable for less polar bioactive molecules like carotenoids. The properties of solvent and extraction temperature are found to be the most important parameters affecting the ASE extraction efficiency of thermolabile natural compounds.
采用加速溶剂萃取（ASE）法从橙色胡萝卜中提取类胡萝卜素，并对萃取参数进行了优化。选择叶黄素和β-胡萝卜素这两种类胡萝卜素作为验证过程。在选择溶剂混合物时考虑了溶剂的希尔德布兰德溶解度参数和介电常数。系统研究了温度、静态时间、干燥剂等各种实验参数对ASE萃取效率的影响。还研究了变量之间的相互作用。此外，通过液相色谱-电喷雾电离质谱法（LC-ESI MS）对两种类胡萝卜素进行了分析和表征。研究得出结论，希尔德布兰德溶解度参数方法可能适用于类胡萝卜素等极性较小的生物活性分子。发现溶剂性质和萃取温度是影响热不稳定天然化合物ASE萃取效率的最重要参数。
