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吞噬性阿米巴(Acanthamoeba polyphaga)内幽门螺杆菌(Helicobacter pylori)的命运:荧光原位杂交和实时聚合酶链反应检测。

The fate of Helicobacter pylori phagocytized by Acanthamoeba polyphaga demonstrated by fluorescent in situ hybridization and quantitative polymerization chain reaction tests.

机构信息

Charlotte Smith & Associates, Inc., PO Box 629, Orinda, CA 94563, USA.

出版信息

Curr Microbiol. 2012 Dec;65(6):805-12. doi: 10.1007/s00284-012-0232-2. Epub 2012 Sep 23.

Abstract

Helicobacter pylori able to express green fluorescent protein, as well as an ATCC strain, and a clinical isolate of this pathogen were evaluated for their ability to survive predation by Acanthamoeba polyphaga. Ingestion was evaluated by microscopic observation of the GFP-H. pylori and BacLight™-stained cells. Following phagocytosis, the fate of cells was assessed by fluorescent in situ hybridization with an oligonucleotide targeting H. pylori 16S rRNA and by quantitative-polymerase chain reaction (qPCR) tests with primers to 16S rDNA. Fluorescent in situ hybridization tests were inconclusive with only a small percentage of amoebae apparently containing active intracellular H. pylori. Furthermore, no increase in bacterial cells was detected by qPCR. Additional research is required to elucidate the mechanisms by which amoebae phagocytize this important bacterial pathogen.

摘要

幽门螺杆菌能够表达绿色荧光蛋白,以及 ATCC 株和该病原体的临床分离株,用于评估它们逃避变形虫多滋养体捕食的能力。通过观察 GFP-幽门螺杆菌和 BacLight 染色细胞来评估摄入。吞噬后,通过针对幽门螺杆菌 16S rRNA 的寡核苷酸进行荧光原位杂交,并通过针对 16S rDNA 的定量聚合酶链反应 (qPCR) 测试来评估细胞的命运。荧光原位杂交测试的结论不一致,只有一小部分变形虫显然含有活性的细胞内幽门螺杆菌。此外,qPCR 未检测到细菌细胞的增加。需要进一步研究阐明变形虫吞噬这种重要的细菌病原体的机制。

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