Department of Chemistry, National Taiwan Normal University, Taipei, Taiwan.
Electrophoresis. 2012 Oct;33(19-20):3073-8. doi: 10.1002/elps.201200270. Epub 2012 Sep 24.
A novel drug-screening system, consisting of paper spray-MS (PS-MS) and a CE-ESI-MS method was developed. This system can be easily switched either to PS-MS for rapidly screening samples or to the traditional CE-ESI-MS method for separation and to obtain detailed mass spectral information, while sharing the same mass spectrometer. In the former case, when a sharp (15°-tip) chromatography paper was used, the optimized distance from the paper tip to the mass inlet was 7.7 mm, whereas the optimized distance for the CE-ESI tip was ∼13.5 mm. Using 4-chloroamphetamine as a model compound, the LODs for PS-MS and CE-ESI-MS were determined to ∼0.1 and 0.25 ppm, respectively. Comparisons of results obtained using PS-MS and CE-ESI-MS and the experimental conditions are described.
一种新型的药物筛选系统，由纸喷雾 - MS（PS-MS）和 CE-ESI-MS 方法组成。该系统可以轻松切换到 PS-MS 进行快速筛选样品，或切换到传统的 CE-ESI-MS 方法进行分离并获得详细的质谱信息，同时共享同一台质谱仪。在前一种情况下，当使用锋利的（15°-尖端）色谱纸时，从纸尖端到质谱入口的最佳距离为 7.7mm，而 CE-ESI 尖端的最佳距离约为 13.5mm。使用 4-氯苯丙胺作为模型化合物，PS-MS 和 CE-ESI-MS 的 LODs 分别确定为约 0.1 和 0.25ppm。描述了使用 PS-MS 和 CE-ESI-MS 获得的结果的比较和实验条件。
