German Institute of Human Nutrition Potsdam-Rehbrücke, Department of Epidemiology, Arthur-Scheunert-Allee 114-116, 14558 Nuthetal, Germany.
Ann Clin Biochem. 2012 Nov;49(Pt 6):542-5. doi: 10.1258/acb.2012.011273. Epub 2012 Sep 21.
Identified as a biomarker of altered calcium-phosphorus metabolism in chronic kidney disease, fibroblast growth factor 23 (FGF-23) can also be used as a biomarker of risk for cardiovascular disease in the general population. However, it is crucial to first evaluate the reproducibility (reliability) of plasma FGF-23 concentrations.
We assessed the reliability of plasma FGF-23 concentrations using replicate blood samples taken four months apart of 207 participants from the European Prospective Investigation into Cancer and Nutrition-Potsdam Study.
Plasma FGF-23 concentrations at baseline (geometric mean: 24.7 RU/mL; 95% confidence interval [CI] in RU/mL: 21.8-27.9) were not significantly different from those measured four months later (geometric mean: 23.7 RU/mL; 95% CI in RU/mL: 20.6-27.1; P = 0.42). The intraclass correlation coefficients were 0.69 (95% CI: 0.62-0.76) for all; 0.64 (95% CI: 0.50-0.75) for men and 0.73 (95% CI: 0.64-0.81) for women.
Plasma FGF-23 concentrations showed good reliability over time. Our findings suggest that in epidemiological studies, a single plasma FGF-23 measurement may be sufficient to derive the relative risk in prospective cohort studies.
成纤维细胞生长因子 23（FGF-23）是慢性肾脏病中钙磷代谢改变的生物标志物，也可用作一般人群心血管疾病风险的生物标志物。然而，首先评估血浆 FGF-23 浓度的可重复性（可靠性）至关重要。
我们使用欧洲癌症前瞻性调查和营养-波茨坦研究的 207 名参与者相隔四个月采集的重复血液样本评估了血浆 FGF-23 浓度的可靠性。
基线时（几何平均值：24.7 RU/mL；RU/mL 的 95%置信区间：21.8-27.9）的血浆 FGF-23 浓度与四个月后测量的浓度（几何平均值：23.7 RU/mL；RU/mL 的 95%置信区间：20.6-27.1；P = 0.42）没有显着差异。总体的组内相关系数为 0.69（95%CI：0.62-0.76）；男性为 0.64（95%CI：0.50-0.75），女性为 0.73（95%CI：0.64-0.81）。
血浆 FGF-23 浓度随时间具有良好的可靠性。我们的发现表明，在流行病学研究中，单次血浆 FGF-23 测量可能足以在前瞻性队列研究中得出相对风险。
