Department of Cell Biology, Microbiology, and Molecular Biology, ISA 2015, University of South Florida, 4202 E. Fowler Avenue, Tampa, Florida 33620, USA.
Am J Bot. 2012 Oct;99(10):e405-7. doi: 10.3732/ajb.1200128. Epub 2012 Sep 21.
To increase the number of variable regions available for phylogenetic study in the Cactaceae, primers were developed for a portion of the plastid ycf1 gene and intron-spanning regions of two low-copy nuclear genes (isi1, nhx1). •
Primers were tested on several families within Caryophyllales, focusing on the Cactaceae. Gel electrophoresis indicated positive amplification in most samples. Sequences of these three regions (isi1, nhx1, ycf1) from Harrisia exhibited variation similar to or greater than two plastid regions (atpB-rbcL intergenic spacer and rpl16 intron). •
The isi, nhx, and ycf1 primers amplify phylogenetically useful information applicable to the Cactaceae and other families in the Caryophyllales.
为了增加仙人掌科植物系统发育研究中可用的可变区数量，开发了针对质体 ycf1 基因部分和两个低拷贝核基因（isi1、nhx1）内含子跨越区的引物。
在 Caryophyllales 中的几个科中测试了这些引物，重点是仙人掌科。凝胶电泳表明大多数样本中都有阳性扩增。从 Harrisia 中获得的这三个区域（isi1、nhx1、ycf1）的序列与两个质体区域（atpB-rbcL 基因间 spacer 和 rpl16 内含子）的相似或更大的变异。
isi、nhx 和 ycf1 引物可扩增适用于仙人掌科和 Caryophyllales 中其他科的系统发育有用信息。
