Université de Lyon, F-69000 Lyon, France, and Laboratoire de Physique, ENS de Lyon, CNRS, F-69007 Lyon, France.
Phys Rev Lett. 2012 Jun 29;108(26):268101. doi: 10.1103/PhysRevLett.108.268101.
Based on an analogy between DNA replication and one dimensional nucleation-and-growth processes, various attempts to infer the local initiation rate I(x,t) of DNA replication origins from replication timing data have been developed in the framework of phase transition kinetics theories. These works have all used curve-fit strategies to estimate I(x,t) from genome-wide replication timing data. Here, we show how to invert analytically the Kolmogorov-Johnson-Mehl-Avrami model and extract I(x,t) directly. Tests on both simulated and experimental budding-yeast data confirm the location and firing-time distribution of replication origins.
基于 DNA 复制和一维成核生长过程之间的类比，在相变动力学理论的框架下，已经有各种尝试从复制时间数据中推断 DNA 复制起始点的局部起始速率 I(x,t)。这些工作都使用曲线拟合策略从全基因组复制时间数据中估计 I(x,t)。在这里，我们展示如何对 Kolmogorov-Johnson-Mehl-Avrami 模型进行解析反演，并直接提取 I(x,t)。对模拟和实验芽殖酵母数据的测试证实了复制起始点的位置和触发时间分布。
