Newlon Carol S, Theis James F
Department of Microbiology and Molecular Genetics, UMDNJ-New Jersey Medical School, Newark 07103, USA.
Bioessays. 2002 Apr;24(4):300-4. doi: 10.1002/bies.10075.
Replication origins, which are responsible for initiating the replication of eukaryotic chromosomal DNAs, are spaced at intervals of 40 to 200 kb. Although the sets of proteins that assemble at replication origins during G(1) to form pre-replicative complexes are highly conserved, the structures of replication origins varies from organism to organism. The identification of replication origins has been a labor-intensive task, requiring the analysis of chromosomal DNA replication intermediates. As a result, only a few replication origins have been identified and studied. In a pair of recently published papers, Raghuraman and colleagues and Wyrick, Aparicio and colleagues provide complementary microarray-based approaches to the identification of replication origins. These genome-wide views of DNA replication in Saccharomyces cerevisiae provide new insights into the way that the genome is duplicated and hold promise for the analysis of other genomes.
负责启动真核染色体DNA复制的复制起点，其间隔为40至200千碱基对。尽管在G(1)期组装在复制起点以形成前复制复合体的蛋白质组高度保守，但复制起点的结构因生物体而异。复制起点的鉴定一直是一项劳动密集型任务，需要分析染色体DNA复制中间体。因此，仅鉴定和研究了少数复制起点。在最近发表的两篇论文中，拉古勒曼及其同事以及怀里克、阿帕里西奥及其同事提供了基于微阵列的互补方法来鉴定复制起点。这些对酿酒酵母DNA复制的全基因组观察为基因组复制方式提供了新见解，并有望用于分析其他基因组。
