Holt M E, Enright M R, Alexander T J
University of Cambridge, Department of Clinical Veterinary Medicine.
Res Vet Sci. 1990 Jan;48(1):23-7.
Ten intravenous inoculations of 10(10) formalin-killed pathogenic Streptococcus suis type 2 stimulated detectable levels of opsonic antibody and a strong protective response against intravenous challenge with 10(9) viable S suis type 2 of the same strain. Heat-killed organisms did not stimulate a protective response, even though the type-specific antigen was intact and opsonic antibodies were induced. When the number of formalin-killed organisms in the inoculum was increased 10-fold, one inoculation was insufficient to stimulate a protective response, but two inoculations protected pigs against intravenous challenge with live, pathogenic organisms. Four-hour and overnight cultures were equally immunogenic, and the response was not enhanced by either Freund's incomplete adjuvant or aluminium hydroxide gel adjuvant.
用10(10)个经福尔马林灭活的致病性2型猪链球菌进行十次静脉接种，可刺激产生可检测水平的调理素抗体，并对静脉注射10(9)个同一菌株的活2型猪链球菌产生强烈的保护性反应。热灭活的细菌虽然其型特异性抗原完整且能诱导调理素抗体，但并未刺激产生保护性反应。当接种物中福尔马林灭活细菌的数量增加10倍时，单次接种不足以刺激产生保护性反应，但两次接种可保护猪免受致病性活细菌的静脉攻击。4小时和过夜培养物的免疫原性相同，弗氏不完全佐剂或氢氧化铝凝胶佐剂均未增强这种反应。
