使用双抗体夹心酶联免疫吸附测定法评估接种2型猪链球菌疫苗的猪的抗体反应。
Evaluation of the antibody response in pigs vaccinated against Streptococcus suis capsular type 2 using a double-antibody sandwich enzyme-linked immunosorbent assay.
作者信息
Blouin C, Higgins R, Gottschalk M, Simard J
机构信息
Département de Pathologie et Microbiologie, Faculté de Médecine vétérinaire, Université de Montréal, Saint-Hyacinthe, Québec.
出版信息
Can J Vet Res. 1994 Jan;58(1):49-54.
Abstract
A double-antibody sandwich enzyme-linked immunosorbent assay (ELISA) was standardized for the detection of specific antibodies following vaccination with Streptococcus suis capsular type 2 bacterins. No statistically significant increase of antibody titers was detected in vaccinated piglets compared to the nonvaccinated control group, even if a minority of piglets demonstrated an important postvaccinal response. Three of four vaccinated sows showed a low antibody response to vaccine and specific immunity was detected in piglets of only one litter of these three sows. Passive protection studies showed that none of the sera from vaccinated piglets were protective for mice whereas serum obtained from hyperimmunized pigs gave protection.
摘要
建立了一种双抗体夹心酶联免疫吸附测定法（ELISA），用于检测接种2型猪链球菌疫苗后产生的特异性抗体。与未接种疫苗的对照组相比，接种疫苗的仔猪抗体滴度没有统计学上的显著增加，即使少数仔猪表现出重要的疫苗接种后反应。四只接种疫苗的母猪中有三只对疫苗的抗体反应较低，仅在这三只母猪中一窝仔猪检测到特异性免疫。被动保护研究表明，接种疫苗的仔猪血清对小鼠均无保护作用，而超免疫猪的血清具有保护作用。
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