通过在液体培养基中连续传代减弱鹅疏螺旋体的毒力。
Attenuation of Borrelia anserina by serial passage in liquid medium.
作者信息
Levine J F, Dykstra M J, Nicholson W L, Walker R L, Massey G, Barnes H J
机构信息
Department of Microbiology, Pathology and Parasitology, North Carolina State University, Raleigh 27606.
出版信息
Res Vet Sci. 1990 Jan;48(1):64-9.
PMID:2300719
Abstract
Borrelia anserina (Sakharoff) was successfully grown in a liquid medium (Barbour-Stoenner-Kelly) for 39 passages. By the 12th serial passage in medium, infectivity of B anserina for chicks was lost. Electron microscopy did not reveal structural differences between non-infective and infective cultured organisms. Changes in the protein profiles were found by electrophoresis as the organisms were passed in culture.
摘要
鹅疏螺旋体（萨哈罗夫）在液体培养基（巴伯-斯托纳-凯利培养基）中成功传代培养了39次。在培养基中传至第12代时，鹅疏螺旋体对雏鸡的感染力丧失。电子显微镜检查未发现非感染性和感染性培养物之间的结构差异。随着培养物传代，通过电泳发现蛋白质谱发生了变化。
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