Hinske Ludwig Christian, Heyn Jens, Galante Pedro A F, Ohno-Machado Lucila, Kreth Simone
Department of Anaesthesiology, Clinic of the University of Munich, Munich, Germany.
Methods Mol Biol. 2013;936:69-76. doi: 10.1007/978-1-62703-083-0_5.
In the recent past, intragenic microRNAs (miRNAs) have gained significant attention. Due to the unique linkage to their host gene's transcription, these miRNAs offer more information than intergenic miRNAs as they associate with some of their hosts' properties. However, genome wide analysis of intronic miRNA data can be very challenging, especially if it relies on Web-based tools only. We therefore describe in this chapter how to set a database and how to link the different publicly available information resources on miRNAs and host genes. We also provide an example of a simple, but useful analysis technique. The basic structures and ideas suggested in this chapter can easily be extended to integrating other data and be applied to different analysis techniques.
最近，基因内微小RNA（miRNA）受到了广泛关注。由于这些miRNA与其宿主基因的转录存在独特的联系，它们与宿主的某些特性相关联，因此比基因间miRNA提供了更多信息。然而，对内含子miRNA数据进行全基因组分析可能非常具有挑战性，尤其是如果仅依赖基于网络的工具。因此，在本章中，我们将描述如何建立一个数据库，以及如何链接关于miRNA和宿主基因的不同公开可用信息资源。我们还提供了一个简单但有用的分析技术示例。本章中提出的基本结构和思路可以很容易地扩展到整合其他数据，并应用于不同的分析技术。
