Department of Medicine/Rheumatology, University of Chicago, Chicago, Illinois 60637, USA.
Nat Rev Immunol. 2012 Oct;12(10):709-19. doi: 10.1038/nri3285.
The natural human antibody response is a rich source of highly specific, neutralizing and self-tolerant therapeutic reagents. Recent advances have been made in isolating and characterizing monoclonal antibodies that are generated in response to natural infection or vaccination. Studies of the human antibody response have led to the discovery of crucial epitopes that could serve as new targets in vaccine design and in the creation of potentially powerful immunotherapies. With a focus on influenza virus and HIV, herein we summarize the technological tools used to identify and characterize human monoclonal antibodies and describe how these tools might be used to fight infectious diseases.
自然人类抗体反应是高度特异性、中和性和自身耐受性治疗试剂的丰富来源。最近在分离和表征针对自然感染或接种疫苗产生的单克隆抗体方面取得了进展。对人类抗体反应的研究导致发现了关键表位，这些表位可以作为疫苗设计和潜在强大免疫疗法的新靶点。本文重点介绍流感病毒和 HIV，我们总结了用于鉴定和表征人类单克隆抗体的技术工具，并描述了如何将这些工具用于对抗传染病。
