Slabbert R, Hepple J-A, Rhode C, Bester-Van der Merwe A E, Roodt-Wilding R
Molecular Aquatic Research Group, Department of Genetics, Stellenbosch University, Matieland, South Africa.
Genet Mol Res. 2012 Aug 24;11(3):2769-79. doi: 10.4238/2012.August.24.2.
Farming of Haliotis midae is the most lucrative aquaculture venture in South Africa. The genome of this species needs to be studied to assist in selective breeding programs aimed at increasing overall yield, and molecular markers will be required to attain this goal. We identified and characterized 82 polymorphic microsatellite loci by using repeat-enriched genomic libraries and high-throughput pyrosequencing technology. The observed number of alleles ranged from 2 to 21, expected heterozygosity from 0.063 to 0.968, observed heterozygosity from 0.000 to 1.000, and polymorphic information content from 0.059 to 0.934. Three loci gave significant hits to other haliotid genes and/or microsatellite loci; hits to genes were always located in the 5'/3'-UTR or intron region. Many of these newly designed markers would be useful for parentage, population and linkage studies.
南非的南非鲍养殖是最赚钱的水产养殖项目。需要对该物种的基因组进行研究，以协助开展旨在提高总产量的选择性育种计划，并且需要分子标记来实现这一目标。我们通过使用富含重复序列的基因组文库和高通量焦磷酸测序技术，鉴定并表征了82个多态性微卫星位点。观察到的等位基因数范围为2至21，预期杂合度为0.063至0.968，观察到的杂合度为0.000至1.000，多态信息含量为0.059至0.934。三个位点与其他鲍属基因和/或微卫星位点有显著匹配；与基因的匹配总是位于5'/3'-非翻译区或内含子区域。许多这些新设计的标记将有助于亲子关系、种群和连锁研究。
