九孔鲍60个微卫星标记的开发与特征分析

Development and characterization of 60 microsatellite markers in the abalone Haliotis diversicolor.

作者信息

Wang Y, Wang F, Shi Y H, Gu Z F, Wang A M

机构信息

Key Laboratory of Tropical Biological Resources of Ministry of Education, Hainan Provincial Key Laboratory of Tropical Hydrobiology and Biotechnology, National Class Marine Biology Experiment Teaching Demonstration Center, Ocean College, Hainan University, Haikou, Hainan, China.

出版信息

Genet Mol Res. 2011 May 10;10(2):860-6. doi: 10.4238/vol10-2gmr1182.

DOI:10.4238/vol10-2gmr1182

PMID:21574142

Abstract

The abalone, Haliotis diversicolor, is one of the most important mariculture species in southern China. We developed 60 new polymorphic microsatellite markers for H. diversicolor and characterized them in 30 individuals from a cultured population in Sanya, China. All 60 markers were found to be polymorphic. The number of alleles ranged from two to nine per locus, with an average of 4.12/locus. The expected and observed heterozygosities ranged from 0.10 to 0.88 and from 0.07 to 0.87, respectively. Forty-four loci were in Hardy-Weinberg equilibrium. These 44 microsatellite markers should be useful for genome mapping and population genetic studies.

摘要

九孔鲍（Haliotis diversicolor）是中国南方最重要的海水养殖物种之一。我们开发了60个新的多态性微卫星标记用于九孔鲍，并在中国三亚的一个养殖群体的30个个体中对其进行了特征分析。发现所有60个标记均具有多态性。每个位点的等位基因数从2个到9个不等，平均每个位点为4.12个。预期杂合度和观察到的杂合度分别在0.10至0.88和0.07至0.87之间。44个位点处于哈迪-温伯格平衡。这44个微卫星标记应有助于基因组作图和群体遗传学研究。