Department of Cardiology, the Red Cross Hospital of Guangzhou City, the Fourth Affiliated Hospital of Jinan University, Guangzhou, People's Republic of China.
J Cardiovasc Pharmacol Ther. 2013 Mar;18(2):133-7. doi: 10.1177/1074248412460123. Epub 2012 Sep 24.
To investigate the expression of β(3)-adrenergic receptor (β(3)-AR) in the atrium of rats with chronic heart failure (CHF).
The heart failure rat model was established by aortic constriction. Thirty-six male Wistar rats were divided into Sham group (n = 10) and heart failure model group (n = 26), which were further divided into CHF control (CHF group) and BRL group. The rats in the BRL group were treated with a selective β(3)-AR agonist, BRL-37344 (4.0 nmol/kg, twice weekly) for 4 weeks.
In the BRL group, the left ventricular end-systolic pressure (83.21 ± 13.0 vs 101.50 ± 12.12 mm Hg) and the absolute values of the maximal rate of rise and fall of left ventricular pressure ([±dP/dtmax] 2.81 ± 0.04 vs 0.35 ± 0.04 and -2.72 ± 0.06 vs -3.33 ± 0.06) were lower than in the CHF group (P < .01). The left atrial mass index (LAMI) in the BRL group (0.4132 ± 0.0306) was higher than that in the CHF (0.3212 ± 0.0136) or Sham group (0.2683 ± 0.0145; P < .01). The levels of the left atrial β(3)-AR messenger RNA (mRNA) expression in the BRL group (0.932 ± 0.055) was higher than that in the CHF (0.706 ± 0.043) or Sham group (0.310 ± 0.020; P < .01). In all animals, there was a positive correlation between the level of β(3)-AR mRNA expression and the left or right atrial mass index (correlation coefficient ranged from 0.744 to 0.937).
There is a significant increase in the β(3)-AR mRNA expression in the atrium of rats with heart failure. The level of β(3)-AR mRNA expression was associated with the AMI and was enhanced by a selective β(3)-AR agonist, BRL-37344.
探讨β（3）-肾上腺素能受体（β（3）-AR）在慢性心力衰竭（CHF）大鼠心房中的表达。
采用主动脉缩窄法建立心衰大鼠模型。将 36 只雄性 Wistar 大鼠分为假手术组（n = 10）和心衰模型组（n = 26），后者再分为心衰对照组（CHF 组）和 BRL 组。BRL 组给予选择性β（3）-AR 激动剂 BRL-37344（4.0 nmol/kg，每周 2 次）治疗 4 周。
BRL 组大鼠左心室收缩末期压（83.21 ± 13.0 比 101.50 ± 12.12mmHg）和左心室压力最大上升和下降速率的绝对值[±dP/dtmax]（2.81 ± 0.04 比 0.35 ± 0.04 和 -2.72 ± 0.06 比 -3.33 ± 0.06）均低于 CHF 组（P <.01）。BRL 组左心房质量指数（LAMI）（0.4132 ± 0.0306）高于 CHF 组（0.3212 ± 0.0136）或假手术组（0.2683 ± 0.0145；P <.01）。BRL 组左心房β（3）-AR 信使 RNA（mRNA）表达水平（0.932 ± 0.055）高于 CHF 组（0.706 ± 0.043）或假手术组（0.310 ± 0.020；P <.01）。在所有动物中，β（3）-AR mRNA 表达水平与左心房或右心房质量指数呈正相关（相关系数范围为 0.744 至 0.937）。
心力衰竭大鼠心房中β（3）-AR mRNA 表达显著增加。β（3）-AR mRNA 表达水平与 AMI 相关，并可被选择性β（3）-AR 激动剂 BRL-37344 增强。
