Department of Chemical Engineering, Worcester Polytechnic Institute, Worcester, MA 01609, USA.
Colloids Surf B Biointerfaces. 2013 Feb 1;102:428-34. doi: 10.1016/j.colsurfb.2012.07.045. Epub 2012 Aug 14.
A method which alters the substrate's physical and electrochemical properties by doping photoresist derived carbon with magnetite nanoparticles has been developed to enhance the existing substrate's ability to foster cell growth. Cyclic voltammetry, scanning electron microscopy and atomic force microscopy are used to evaluate the characters of the prepared film. And then, the magnetite nanoparticles doped carbon film is used as substrate for the growth of nerve cell. Here, rat pheochromocytoma cells are used for culture to test substrate-cell interactions. The results showed an increase in cell concentration and average neurite length with the increase of nanoparticle concentration on the surface. Importantly, the nerve cells can be grown on the magnetite nanoparticles doped carbon even in the absence of nerve growth factor. This finding will potentially provide a new material for nerve regeneration.
一种通过掺杂带有磁铁矿纳米粒子的光致抗蚀剂来改变基底物理和电化学性质的方法已经被开发出来，以增强现有基底促进细胞生长的能力。循环伏安法、扫描电子显微镜和原子力显微镜用于评估所制备薄膜的特性。然后，将掺杂有磁铁矿纳米粒子的碳薄膜用作神经细胞生长的基底。在这里，使用大鼠嗜铬细胞瘤细胞进行培养以测试基底-细胞相互作用。结果表明，随着表面纳米粒子浓度的增加，细胞浓度和平均神经突长度增加。重要的是，即使没有神经生长因子，神经细胞也可以在掺杂有磁铁矿纳米粒子的碳上生长。这一发现可能为神经再生提供一种新材料。
