Institute of Life Science, Scuola Superiore Sant'Anna, Pisa, Italy.
Instituto de Nanociencia de Aragón, Universidad de Zaragoza, Mariano Esquillor, Zaragoza, Spain; Departamento de Física de la Materia Condensada, Pedro Cerbuna, Zaragoza, Spain.
Nanomedicine. 2014 Oct;10(7):1549-58. doi: 10.1016/j.nano.2013.12.008. Epub 2014 Jan 7.
There is a growing body of evidence indicating the importance of physical stimuli for neuronal growth and development. Specifically, results from published experimental studies indicate that forces, when carefully controlled, can modulate neuronal regeneration. Here, we validate a non-invasive approach for physical guidance of nerve regeneration based on the synergic use of magnetic nanoparticles (MNPs) and magnetic fields (Ms). The concept is that the application of a tensile force to a neuronal cell can stimulate neurite initiation or axon elongation in the desired direction, the MNPs being used to generate this tensile force under the effect of a static external magnetic field providing the required directional orientation. In a neuron-like cell line, we have confirmed that MNPs direct the neurite outgrowth preferentially along the direction imposed by an external magnetic field, by inducing a net angle displacement (about 30°) of neurite direction. From the clinical editor: This study validates that non-invasive approaches for physical guidance of nerve regeneration based on the synergic use of magnetic nanoparticles and magnetic fields are possible. The hypothesis was confirmed by observing preferential neurite outgrowth in a cell culture system along the direction imposed by an external magnetic field.
越来越多的证据表明物理刺激对于神经元的生长和发育很重要。具体来说，已发表的实验研究结果表明，在精心控制的情况下，力可以调节神经元的再生。在这里，我们验证了一种基于磁纳米粒子（MNPs）和磁场（Ms）协同使用的非侵入性物理神经再生引导方法。其概念是，对神经元细胞施加张力可以刺激所需方向的轴突起始或轴突伸长，MNPs 用于在静态外部磁场的作用下产生这种张力，从而提供所需的定向方向。在神经元样细胞系中，我们已经证实 MNPs 通过诱导神经突方向的净角度位移（约 30°），优先沿着外部磁场施加的方向引导神经突生长。临床编辑按：本研究证实了基于磁纳米粒子和磁场协同使用的非侵入性物理神经再生引导方法是可行的。该假设通过观察细胞培养系统中沿着外部磁场施加的方向优先进行神经突生长得到了证实。
