Laboratory of Integrated Pest Management in Crops, Ministry of Agriculture, Institute of Plant Protection, Chinese Academy of Agricultural Sciences, Beijing, 100193, China.
Gene. 2012 Dec 15;511(2):265-73. doi: 10.1016/j.gene.2012.09.027. Epub 2012 Sep 23.
Ryanodine receptors (RyRs), members of the largest family of calcium channel proteins, have been studied because of their key roles in calcium signalling within cells. With the development of diamide insecticides that exhibit a novel mode of action on the RyRs from Lepidoptera, research on insect RyRs has become more attractive in the field of plant protection. To enhance our understanding of the effects of diamides on RyRs, we cloned the Plutella xylostella RyR gene (Px-RyR), which is the most serious pest of Brassicaceae plants throughout the world. Furthermore, we investigated the modulation of the expression of Px-RyR as a result of the application of diamide insecticides. The full-length cDNAs of Px-RyR contain an open reading frame (ORF) of 15,372bp with a predicted protein consisting of 5123 amino acids. Px-RyR possesses a high level of overall amino acid homology with other isoforms (77-92% identity with insect isoforms and 45-47% identity with vertebrate isoforms). The weight of Px. gradually decreased as the concentration of the diamides increased. However, the relative expression levels of the RyRs from larvae were dependent on the insecticide concentration and gradually increased with increasing insecticide concentrations.
肌质网钙释放通道（Ryanodine receptors，RyRs）是钙通道蛋白家族中最大的成员之一，因其在细胞内钙信号传递中的关键作用而受到研究。随着具有新型作用模式的二酰胺类杀虫剂在鳞翅目昆虫 RyRs 上的发展，昆虫 RyRs 的研究在植物保护领域变得更具吸引力。为了增强我们对二酰胺类杀虫剂对 RyRs 影响的理解，我们克隆了小菜蛾 RyR 基因（Px-RyR），该基因是全世界十字花科植物最严重的害虫。此外，我们还研究了二酰胺类杀虫剂对 Px-RyR 表达的调控。Px-RyR 的全长 cDNA 包含一个 15372bp 的开放阅读框（ORF），预测的蛋白质由 5123 个氨基酸组成。Px-RyR 与其他同工型具有高度的整体氨基酸同源性（与昆虫同工型的同一性为 77-92%，与脊椎动物同工型的同一性为 45-47%）。随着二酰胺类浓度的增加，Px 的重量逐渐降低。然而，幼虫 RyRs 的相对表达水平取决于杀虫剂浓度，并随着杀虫剂浓度的增加而逐渐增加。
