State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China.
Bioresour Technol. 2013 May;135:574-7. doi: 10.1016/j.biortech.2012.08.120. Epub 2012 Sep 4.
Efficient biosynthesis of succinate from a renewable biomass resource by engineered Escherichia coli is reported in this paper. Fermentation of sugarcane bagasse hydrolysate by engineered E. coli BA204, a pflB, ldhA, and ppc deletion strain overexpressing the ATP-forming phosphoenolpyruvate carboxykinase from Bacillus subtilis 168, produced a final succinate concentration of 15.85 g L(-1) with a high yield of 0.89 g L(-1) total sugar under anaerobic conditions. During dual-phase fermentations, initial aerobic growth facilitated subsequent anaerobic succinate production, with a final succinate concentration of 18.88 g L(-1) and a yield of 0.96 g g(-1) total sugar after 24 h of anaerobic fermentation. The high succinate yield from sugarcane bagasse hydrolysate demonstrated a great potential application of renewable biomass as a feedstock for the economical production of succinate using metabolically engineered E. coli.
本文报道了利用工程化大肠杆菌从可再生生物质资源高效合成琥珀酸。通过对过表达枯草芽孢杆菌 168 中的 ATP 形成磷酸烯醇丙酮酸羧激酶的 pflB、ldhA 和 ppc 缺失菌株工程大肠杆菌 BA204 进行发酵，利用甘蔗渣水解液在厌氧条件下最终产生了 15.85g/L 的琥珀酸，总糖得率为 0.89g/L。在两相发酵过程中，初始有氧生长促进了随后的厌氧琥珀酸生产，经过 24 小时的厌氧发酵，最终琥珀酸浓度为 18.88g/L，总糖得率为 0.96g/g。从甘蔗渣水解液中获得的高琥珀酸产量表明，可再生生物质作为经济生产琥珀酸的原料，利用代谢工程大肠杆菌具有很大的潜在应用价值。
