State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing University of Technology, Nanjing 211816, China.
Bioresour Technol. 2013 Sep;143:405-12. doi: 10.1016/j.biortech.2013.06.031. Epub 2013 Jun 17.
In this study, repetitive production of succinic acid from lignocellulose hydrolysates by enhancement of ATP supply in metabolically engineered E. coli is reported. Escherichia coli BA305, a pflB, ldhA, ppc, and ptsG deletion strain overexpressing ATP-forming phosphoenolpyruvate (PEP) carboxykinase (PEPCK), produced a final succinic acid concentration of 83 g L(-1) with a high yield of 0.87 g g(-1) total sugar in 36 h of three repetitive fermentations of sugarcane bagasse hydrolysate. Furthermore, simultaneous consumption of glucose and xylose was achieved, and the specific productivity and yield of succinic acid were almost maintained constant during the repetitive fermentations.
本研究通过增强代谢工程大肠杆菌中 ATP 的供应，从木质纤维素水解物中重复生产琥珀酸。产酸克雷伯氏菌 BA305 是一株 pflB、ldhA、ppc 和 ptsG 缺失株，过表达形成 ATP 的磷酸烯醇丙酮酸（PEP）羧激酶（PEPCK），在重复发酵甘蔗渣水解物 36 小时内，最终琥珀酸浓度达到 83 g/L，总糖得率为 0.87 g/g。此外，实现了葡萄糖和木糖的同时消耗，在重复发酵过程中，琥珀酸的比生产力和得率几乎保持不变。
