Bao Haijiao, Liu Rongming, Liang Liya, Jiang Youming, Jiang Min, Ma Jiangfeng, Chen Kequan, Jia Honghua, Wei Ping, Ouyang Pingkai
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
State Key Laboratory of Materials-Oriented Chemical Engineering, College of Biotechnology and Pharmaceutical Engineering, Nanjing Tech University, Nanjing 211816, China.
Enzyme Microb Technol. 2014 Nov;66:10-5. doi: 10.1016/j.enzmictec.2014.04.017. Epub 2014 Jul 18.
Atmospheric and room temperature plasma and adaptive evolution were combined to generate Escherichia coli mutants, which can simultaneously and efficiently utilize glucose and xylose to produce succinic acid in chemically defined medium under exclusively anaerobic condition. Compared to the parent strain BA305, a pflB, ldhA, ppc, and ptsG deletion strain overexpressing ATP-forming phosphoenolpyruvate (PEP) carboxykinase (PEPCK), the sugar consumption rate and succinic acid productivity of mutant BA408 were significantly improved with a marked increase in the key enzyme activities. Subsequent anaerobic fermentation of BA408 with corn stalk hydrolysate produced a final succinic acid concentration of 23.1 g L(-1) with a yield of 0.85 g g(-1) sugar mixture. The observed synthesis of succinic acid from the corn stalk hydrolysate showed a great potential usage of renewable biomass as a feedstock for an economical succinic acid production using E. coli.
将常压和室温等离子体与适应性进化相结合，以产生大肠杆菌突变体，该突变体能够在完全厌氧条件下的化学限定培养基中同时高效利用葡萄糖和木糖来生产琥珀酸。与过表达生成ATP的磷酸烯醇丙酮酸（PEP）羧激酶（PEPCK）的pflB、ldhA、ppc和ptsG缺失菌株亲本菌株BA305相比，突变体BA408的糖消耗率和琥珀酸生产率显著提高，关键酶活性明显增加。随后用玉米秸秆水解物对BA408进行厌氧发酵，最终琥珀酸浓度为23.1 g L(-1)，产率为0.85 g g(-1)糖混合物。从玉米秸秆水解物中观察到的琥珀酸合成表明，使用大肠杆菌将可再生生物质作为原料用于经济地生产琥珀酸具有巨大的潜在用途。
