Amino acid-based tweezers: the role of turn-like conformation in the binding of copper(II).

The importance of turn-like peptide conformation for the copper(II) binding has been revealed by the synthesis of simple amino acid-based tweezers and the study of their interaction with copper(II). Amino acids Phe, Leu, Val, Ala and Gly were bridged through their C-terminuses with conformationally constrained motif, cis enediyne moiety ((Z)-octa-4-en-2,6-diyne-1,8-diamine). The interaction of prepared diamine ligands with copper(II) was studied by means of potentiometric titrations, UV-visible and EPR spectroscopic and mass spectrometric techniques. All ligands interact efficiently with copper(II) and form complexes of 1:1 stoichiometry differing in the protonation state of the ligand. LCu(2+) species were found predominant at pH<6.5, with log K* ranging from -8.06 to -6.65, while at higher pH deprotonation occurred, giving rise to LH(-1)Cu(+) complexes or LH(-2)Cu complex for the phenylalanine-related ligand. An additional species, LH(-3)Cu(-) were found at pH>9 for the valine- and alanine-related ligands, respectively. Comparing stability of studied complexes with those reported in previous work revealed that ligands effectively emulate properties of copper(II) binding peptides. Based on the results obtained in this work it can be concluded that structural rigidity significantly enhances coordination properties of the ligand, thus conforming importance of the turn-like peptide conformation for the copper(II) binding.