Division of Biological Science and Technology, Yonsei University, Wonju 220-100, Republic of Korea.
FEBS Lett. 2012 Oct 19;586(20):3753-60. doi: 10.1016/j.febslet.2012.09.010. Epub 2012 Sep 23.
Replication protein A (RPA) is a eukaryotic single-stranded DNA binding protein that is essential for DNA replication, repair, and recombination, and human RPA interacting protein α (hRIPα) is the nuclear transporter of RPA. Here, we report the regulatory role of hRIPα protein in cell proliferation. Western blot analysis revealed that the level of hRIPα was frequently elevated in cervical tumors tissues and hRIPα knockdown by siRNA inhibited cellular proliferation through deregulation of the cell cycle. In addition, overexpression of hRIPα resulted in increased clonogenicity. These results indicate that hRIPα is involved in cell proliferation through regulation of RPA transport.
复制蛋白 A（RPA）是一种真核生物单链 DNA 结合蛋白，对于 DNA 的复制、修复和重组至关重要，人类 RPA 相互作用蛋白 α（hRIPα）是 RPA 的核转运蛋白。在这里，我们报告了 hRIPα 蛋白在细胞增殖中的调节作用。Western blot 分析显示，hRIPα 水平在宫颈肿瘤组织中经常升高，而通过 siRNA 敲低 hRIPα 可通过细胞周期失调抑制细胞增殖。此外，hRIPα 的过表达导致集落形成能力增加。这些结果表明，hRIPα 通过调节 RPA 运输参与细胞增殖。
