Tomada Inês, Fernandes Dalila, Guimarães João Tiago, Almeida Henrique, Neves Delminda
Department of Experimental Biology, Faculty of Medicine and Instituto de Biologia Molecular e Celular (IBMC) of Universidade do Porto, Alameda Professor Hernâni Monteiro, 4200-319, Porto, Portugal,
Age (Dordr). 2013 Oct;35(5):1721-39. doi: 10.1007/s11357-012-9473-z. Epub 2012 Sep 26.
High-fat (HF) diet regular intake along life highly contributes to vascular dysfunction and to an increment in prevalence of metabolic syndrome (MetS) and erectile dysfunction (ED), a surrogate symptom of occult vascular disease, in the elderly. However, little is known about the effects of energy restriction (ER) alone/or after an HF-feeding period. We show here that in male Sprague-Dawley rats, 16 months of HF-diet consumption led to an increase in body adiposity, blood pressure, lipidemia, C-reactive protein, and insulin resistance and to hypoadiponectinemia, conditions that cluster in MetS. In addition, this treatment strongly favored collagen deposition in cavernous tissue and myocardium. Conversely, for the same time period, the ingestion of 75 % of ad libitum energy intake by controls (ER) extensively counteracted these outcomes. The impact of 6-month ER after 10-month HF period was also analyzed, and despite the decrease in body weight, adiposity, blood pressure, lipidemia, and C-reactive protein and improvement of insulin sensitivity, no differences were observed either in adiponectin blood levels or in retroperitoneal fat pad mass. Moreover, this treatment led to a reduction in cavernous tissue collagen deposition, but not in the myocardium, and evidenced differential mobilization of adipose tissue accretions. The data show the ability of HF diet to cause MetS and produce unwanted effects on myocardium and corpora vascular structure. They also indicate that these consequences are preventable upon ER diet starting early, but not later, in life.
长期规律摄入高脂（HF）饮食会极大地导致老年人血管功能障碍，并增加代谢综合征（MetS）和勃起功能障碍（ED）的患病率，勃起功能障碍是隐匿性血管疾病的一种替代症状。然而，关于单独的能量限制（ER）或在高脂喂养期后的影响，我们所知甚少。我们在此表明，在雄性Sprague-Dawley大鼠中，16个月的高脂饮食消耗导致身体肥胖、血压、血脂、C反应蛋白和胰岛素抵抗增加，以及脂联素血症降低，这些情况都聚集在代谢综合征中。此外，这种处理强烈促进海绵体组织和心肌中的胶原蛋白沉积。相反，在同一时间段内，对照组摄入自由采食量75%的能量（ER）广泛抵消了这些结果。我们还分析了在10个月高脂期后进行6个月能量限制的影响，尽管体重、肥胖、血压、血脂和C反应蛋白有所下降，胰岛素敏感性有所改善，但脂联素血水平或腹膜后脂肪垫质量均未观察到差异。此外，这种处理导致海绵体组织胶原蛋白沉积减少，但心肌中未减少，并证明了脂肪组织积聚的不同动员情况。数据显示高脂饮食会导致代谢综合征，并对心肌和海绵体血管结构产生不良影响。它们还表明，这些后果在生命早期而非晚期开始进行能量限制饮食时是可以预防的。
