College of Biological Industry, Chengdu University, Chengdu 610106, Sichuan, China.
Molecules. 2012 Sep 25;17(10):11335-45. doi: 10.3390/molecules171011335.
The purpose of this study was to investigate the effects of yeast polysaccharide (YPS) on growth and flavonoid accumulation in sprout cultures of Fagopyrum tataricum (tartary buckwheat). Without obvious change in the appearance of the sprouts, the exogenous YPS notably stimulated the production of functional metabolites in F. tataricum sprouts, and the stimulation effect was concentration-dependent. With 400 mg/L of YPS applied to the sprout cultures on day 6, the total rutin and quercentin content was effectively increased to 42.8 mg/gdw, or about 1.4-fold in comparison with the control of 31.2 mg/gdw. Feeding with 800 mg/L of YPS on day 9, the sprouts biomass was increased by about 8% compared to the control culture (0.99 gdw/100 sprouts versus 0.92 gdw/100 sprouts). Moreover, the present study revealed that the accumulation of these bioactive metabolites resulted from the stimulation of the phenylpropanoid pathway by YPS treatment. It could be an effective strategy for improving the functional quality of the F. tataricum sprouts provided with YPS.
本研究旨在探讨酵母多糖（YPS）对苦荞芽生长和类黄酮积累的影响。外源性 YPS 对苦荞芽的功能性代谢产物的产生有明显的促进作用，且这种促进作用呈浓度依赖性。在第 6 天向芽培养物中添加 400 mg/L 的 YPS 时，总芦丁和槲皮素含量有效增加至 42.8 mg/gdw，与对照组的 31.2 mg/gdw 相比约增加了 1.4 倍。在第 9 天向芽培养物中添加 800 mg/L 的 YPS 时，与对照组相比，芽生物量增加了约 8%（0.99 gdw/100 芽对 0.92 gdw/100 芽）。此外，本研究表明，YPS 处理通过刺激苯丙素途径促进了这些生物活性代谢物的积累。YPS 的添加为改善苦荞芽的功能品质提供了一种有效策略。
