Bakhvalov S A, Martem'ianov V V, Bakhvalova V N, Morozova O V
Vopr Virusol. 2012 Jul-Aug;57(4):35-7.
The nuclear polyhedrosis virus (NPV) DNA was detected in samples from eggs and caterpillars of the gypsy moth collected in natural populations of the Western Siberia and Ural by means of PCR with primers corresponding to the polyhedrin gene. According to censuring data, the gypsy moth populations of Western Siberia were at the depression stage. The NPV DNA detection frequencies in eggs (8.6 +/- 4.8% - 13.6 +/- 5.2%) and caterpillars (21.0 +/- 6.3% - 22.2 +/- 6.7%) were not significantly differed. In the Urals, collection of the insects was performed in their gradation focus at the phase of maximal abundance. The DNA detection rate in eggs (11.4 +/- 5.0%) was confidently (p < 0.001) lower than in caterpillars (59.8 +/- 5.6%). Consequently, variations of the NPV infection prevalence during ontogenesis of Lymantria dispar (L.) was associated with the gradation cycle of the insect population dynamics.
利用与多角体蛋白基因对应的引物，通过聚合酶链式反应（PCR），在西西伯利亚和乌拉尔自然种群中采集的舞毒蛾卵和幼虫样本中检测到了核型多角体病毒（NPV）DNA。根据监测数据，西西伯利亚的舞毒蛾种群处于衰退阶段。卵（8.6±4.8% - 13.6±5.2%）和幼虫（21.0±6.3% - 22.2±6.7%）中NPV DNA的检测频率无显著差异。在乌拉尔地区，昆虫采集是在其数量最多阶段的分级聚集点进行的。卵中DNA的检测率（11.4±5.0%）显著低于幼虫（59.8±5.6%）（p < 0.001）。因此，舞毒蛾个体发育过程中NPV感染率的变化与昆虫种群动态的分级周期有关。
