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[舞毒蛾核型多角体病毒分离株的比较特征]

[Comparative characteristics of nuclear polyhedrosis virus isolates from gypsy moth Lymantria dispar L].

作者信息

Gorbunova E E, Kolosov A V, Borisova O D, Zaĭstev B N, Bozhko N A

出版信息

Vopr Virusol. 1997 Jan-Feb;42(1):41-3.

PMID:9103045
Abstract

Eight strains of Gypsy moth nuclear polyhedrosis virus (NPV) were isolated from dead larvae in Kirghizia and Tyumen and Novosibirsk districts. The strains were characterized in terms of polyhedron morphology, biological activity, virus production, and restriction analysis of DNA. The isolated strains did not differ in size or shape of polyhedrons. NPV strain NSHN-2 was characterized by the highest biological activity (LD50 = 1.2 +/- 0.4) x 10(4) and maximum production: 7.3 x 10(8) PIB/larva in comparison with the rest isolated strains. This strain is proposed for the manufacture of an insecticide.

摘要

从吉尔吉斯斯坦以及秋明州和新西伯利亚地区的死幼虫中分离出了8株舞毒蛾核型多角体病毒(NPV)。对这些毒株在多角体形态、生物活性、病毒产量以及DNA限制性分析等方面进行了鉴定。分离出的毒株在多角体的大小或形状上没有差异。与其他分离出的毒株相比,NPV毒株NSHN - 2具有最高的生物活性(LD50 = 1.2 +/- 0.4)×10⁴,且产量最高:7.3×10⁸多角体包涵体/幼虫。建议用该毒株生产杀虫剂。

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