Department of Orthopaedics, Shanghai Sixth People's Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, P.R. China.
Tissue Eng Part A. 2013 Feb;19(3-4):529-37. doi: 10.1089/ten.TEA.2012.0208. Epub 2012 Nov 2.
Physical barriers are commonly used to reduce peritendinous adhesion after injury. However, the inflammatory response to surgery cannot be prevented. This study was designed to evaluate the ability of ibuprofen-loaded poly(l-lactic acid)-polyethylene glycol (PELA) diblock copolymer fibrous membranes in preventing adhesion formation and reduce inflammation. Electrospun PELA fibrous membranes underwent mechanical testing and were characterized by morphology, surface wettability, drug release, and degradation. Results of an in vitro drug release study showed that a burst release was followed by sustained release from fibrous membranes with high initial ibuprofen content. Fewer L929 mouse fibroblasts adhered to and proliferated on the ibuprofen-loaded PELA fibrous membrane compared with tissue culture plates or PELA fibrous membrane without ibuprofen. In a chicken model of flexor digitorum profundus tendon surgery, the ibuprofen-loaded PELA fibrous membranes prevented tissue adhesion and significantly reduced inflammation. Taken together, these results demonstrate that ibuprofen-loaded PELA fibrous membranes prevent peritendinous adhesion formation better than membranes that do not contain ibuprofen, through anti-adhesion and anti-inflammatory actions.
物理屏障通常用于减少损伤后腱周粘连。然而，手术引起的炎症反应无法预防。本研究旨在评估载布洛芬的聚（L-丙交酯）-聚乙二醇（PELA）两亲嵌段共聚物纤维膜在预防粘连形成和减轻炎症方面的能力。静电纺 PELA 纤维膜进行了机械性能测试，并通过形态、表面润湿性、药物释放和降解进行了表征。体外药物释放研究结果表明，具有高初始布洛芬含量的纤维膜呈现出初始突释后持续释放。与组织培养板或不含布洛芬的 PELA 纤维膜相比，负载布洛芬的 PELA 纤维膜上的 L929 小鼠成纤维细胞的黏附和增殖较少。在鸡屈肌腱深屈肌腱手术模型中，载布洛芬的 PELA 纤维膜可防止组织粘连，并显著减轻炎症。综上所述，这些结果表明，载布洛芬的 PELA 纤维膜通过抗粘连和抗炎作用，比不含布洛芬的膜更能预防腱周粘连的形成。
