Timlin J A, Carden A, Morris M D, Bonadio J F, Hoffler C E, Kozloff K M, Goldstein S A
J Biomed Opt. 1999 Jan;4(1):28-34. doi: 10.1117/1.429918.
Hyperspectral Raman images of mineral components of trabecular and cortical bone at 3 μm spatial resolution are presented. Contrast is generated from Raman spectra acquired over the 600-1400 cm-1 Raman shift range. Factor analysis on the ensemble of Raman spectra is used to generate descriptors of mineral components. In trabecular bone independent phosphate (PO4-3) and monohydrogen phosphate (HPO4-2) factors are observed. Phosphate and monohydrogen phosphate gradients extend from trabecular packets into the interior of a rod. The gradients are sharply defined in newly regenerated bone. There, HPO4-2 content maximizes near a trabecular packet and decreases to a minimum value over as little as a 20 μm distance. Incomplete mineralization is clearly visible. In cortical bone, factor analysis yields only a single mineral factor containing both PO4-3 and HPO4-2 signatures and this implies uniform distribution of these ions in the region imaged. Uniform PO4-3 and HPO4-2 distribution is verified by spectral band integration. © 1999 Society of Photo-Optical Instrumentation Engineers.
展示了在3μm空间分辨率下小梁骨和皮质骨矿物质成分的高光谱拉曼图像。对比度由在600 - 1400 cm-1拉曼位移范围内采集的拉曼光谱产生。对拉曼光谱集合进行因子分析以生成矿物质成分的描述符。在小梁骨中观察到独立的磷酸盐（PO4-3）和磷酸一氢盐（HPO4-2）因子。磷酸盐和磷酸一氢盐梯度从小梁束延伸到杆状结构内部。这些梯度在新再生骨中定义清晰。在那里，HPO4-2含量在小梁束附近达到最大值，并在仅20μm的距离内降至最小值。明显可见矿化不完全。在皮质骨中，因子分析仅产生一个包含PO4-3和HPO4-2特征的单一矿物质因子，这意味着这些离子在所成像区域内分布均匀。通过光谱带积分验证了PO4-3和HPO4-2的均匀分布。© 1999 光电仪器工程师协会。
