体内追踪标记细胞的技术：X/Y荧光原位杂交的应用、优化荧光检测的技术以及β-半乳糖苷酶检测。
Techniques for following labeled cells in vivo: use of X/Y FISH, techniques to optimize fluorescent detection, and beta-galactosidase detection.
作者信息
Craig Michael, Schumacher Michael, Zavros Yana
机构信息
Department of Molecular and Cellular Physiology, University of Cincinnati College of Medicine, Cincinnati, OH, USA.
出版信息
Methods Mol Biol. 2012;921:227-38. doi: 10.1007/978-1-62703-005-2_25.
The redistribution and trafficking patterns of cells to different anatomic sites throughout the body is important during cancer development and metastasis. Interest in the origin and fate of gastric cancer stem cells has recently arisen, as it may explain the underlying mechanism of cancer development. The ability to monitor the migration patterns of cancer stem cells is imperative to understanding the functional changes associated with the migration and proliferation of these cells. Here we detail a collection of techniques that include fluorescent in vivo imaging, X/Y FISH, and beta-galactosidase detection that are used for following labeled cells in vivo after adoptive transfer or transplant of donor cells for identifying the migration and engraftment of donor cells within the recipient.
在癌症发展和转移过程中，细胞向全身不同解剖部位的重新分布和运输模式至关重要。最近人们对胃癌干细胞的起源和命运产生了兴趣，因为这可能解释癌症发展的潜在机制。监测癌症干细胞迁移模式的能力对于理解与这些细胞迁移和增殖相关的功能变化至关重要。在这里，我们详细介绍了一系列技术，包括荧光体内成像、X/Y荧光原位杂交和β-半乳糖苷酶检测，这些技术用于在过继转移或移植供体细胞后在体内追踪标记细胞，以确定供体细胞在受体中的迁移和植入情况。
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