Fontenete Silvia, Leite Marina, Figueiredo Ceu, Cos Paul, Azevedo Nuno F
LEPABE, Laboratory for Process Engineering, Environment, Biotechnology and Energy, Department of Chemical Engineering, Faculty of Engineering, University of Porto, Porto, Portugal.
i3s Instituto de Investigação e Inovação em Saúde, Universidade do Porto, Porto, Portugal.
Methods Mol Biol. 2017;1616:137-146. doi: 10.1007/978-1-4939-7037-7_8.
In this chapter, we describe a fluorescence in vivo hybridization (FIVH) protocol, using nucleic acid probes, for the detection of the bacterium Helicobacter pylori in the gastric mucosa of an infected C57BL/6 mouse model. This protocol should be easily extended to other microorganisms not only as a way to identify in vivo important microorganisms and their patterns of distribution within specific or at different anatomic sites, but also to better understand interaction mechanisms involving the microbiome and the human body.
在本章中，我们描述了一种使用核酸探针的荧光体内杂交（FIVH）方案，用于在感染的C57BL/6小鼠模型的胃黏膜中检测幽门螺杆菌。该方案应易于扩展到其他微生物，不仅作为一种识别体内重要微生物及其在特定或不同解剖部位的分布模式的方法，还能更好地理解涉及微生物群与人体的相互作用机制。
