Neuroendocrine Unit, IMBICE (CONICET-CICPBA), 1900 La Plata, Argentina.
Nutrients. 2012 Aug;4(8):1137-50. doi: 10.3390/nu4081137. Epub 2012 Aug 22.
The effect of progesterone (P4) on fructose rich diet (FRD) intake-induced metabolic, endocrine and parametrial adipose tissue (PMAT) dysfunctions was studied in the adult female rat. Sixty day-old rats were i.m. treated with oil alone (control, CT) or containing P4 (12 mg/kg). Rats ate Purina chow-diet ad libitum throughout the entire experiment and, between 100 and 120 days of age drank ad libitum tap water alone (normal diet; CT-ND and P4-ND) or containing fructose (10% w/v; CT-FRD and P4-FRD). At age 120 days, animals were subjected to a glucose tolerance test or decapitated. Plasma concentrations of various biomarkers and PMAT gene abundance were monitored. P4-ND (vs. CT-ND) rats showed elevated circulating levels of lipids. CT-FRD rats displayed high (vs. CT-ND) plasma concentrations of lipids, leptin, adiponectin and plasminogen activator inhibitor-1 (PAI-1). Lipidemia and adiponectinemia were high (vs. P4-ND) in P4-FRD rats. Although P4 failed to prevent FRD-induced hyperleptinemia, it was fully protective on FRD-enhanced plasma PAI-1 levels. PMAT leptin and adiponectin mRNAs were high in CT-FRD and P4-FRD rats. While FRD enhanced PMAT PAI-1 mRNA abundance in CT rats, this effect was absent in P4 rats. Our study supports that a preceding P4-enriched milieu prevented the enhanced prothrombotic risk induced by FRD-elicited high PAI-1 production.
孕激素（P4）对富含果糖饮食（FRD）摄入引起的代谢、内分泌和子宫旁脂肪组织（PMAT）功能障碍的影响在成年雌性大鼠中进行了研究。60 日龄大鼠肌肉内注射单独的油（对照组，CT）或含有 P4（12 mg/kg）。在整个实验过程中，大鼠自由摄入 Purina 普通饲料，100 至 120 日龄时自由饮用单独的自来水（正常饮食；CT-ND 和 P4-ND）或含有果糖（10%w/v；CT-FRD 和 P4-FRD）。在 120 日龄时，动物接受葡萄糖耐量试验或断头。监测各种生物标志物和 PMAT 基因丰度的血浆浓度。P4-ND（与 CT-ND 相比）大鼠显示循环脂质水平升高。CT-FRD 大鼠显示高（与 CT-ND 相比）血浆脂质、瘦素、脂联素和纤溶酶原激活物抑制剂-1（PAI-1）浓度。在 P4-FRD 大鼠中，脂质血症和脂联素血症升高（与 P4-ND 相比）。尽管 P4 未能预防 FRD 诱导的高瘦素血症，但它对 FRD 增强的血浆 PAI-1 水平具有完全的保护作用。PMAT 瘦素和脂联素 mRNA 在 CT-FRD 和 P4-FRD 大鼠中均升高。虽然 FRD 增强了 CT 大鼠的 PMAT PAI-1 mRNA 丰度，但在 P4 大鼠中则没有。我们的研究表明，先前富含 P4 的环境可防止 FRD 引起的高 PAI-1 产生所引起的增强的血栓形成风险。
