Song Guang-Qing, Xi Hong-Bo, Zhou Yue-Xi, Li Jie, Zhao Jing-Tian, Cui Jun-Hua
Research Center of Water Pollution Control Technology, Chinese Research Academy of Environmental Sciences, Beijing 100012, China.
Guang Pu Xue Yu Guang Pu Fen Xi. 2012 Jul;32(7):1838-41.
In view of synchronous fluorescence possessing the character of good selectivity, high sensitivity, less interference, etc. it can be used for simultaneous determination of multi-component mixtures of polycyclic aromatic hydrocarbons (PAHs). A new method of constant-wavelength synchronous fluorescence spectrometry to determine two naphthalene and phenanthrene of PAHs was developed in this study. The effect of different experimental conditions, such as different disolvents for character of fluorescence spectra and the choose of the optimal wavelength difference were studied. Experiment showed that the simultaneous indentification and quantitative determination of the two PAHs when delta lambda = 100 was chosen. The fluorescence intensity was linearly related to naphthalene and phenanthrene concentration in the range of 0.5-25.0 microg x L(-1) with correlation coefficient 0.999 5 and 0.999 7, respectively. The detection limits were all lower than 0.03 microg x L(-1), and the relative standard deviations for naphthalene and phenanthrene were 1.19% and 180% (n=7), respectively. Results show that the compounds can be analyzed qualitatively and quantitatively by synchronous fluorescence spectrometry.
鉴于同步荧光具有选择性好、灵敏度高、干扰少等特点，可用于多环芳烃（PAHs）多组分混合物的同时测定。本研究建立了一种恒波长同步荧光光谱法测定PAHs中萘和菲的新方法。研究了不同实验条件的影响，如不同溶剂对荧光光谱特性的影响以及最佳波长差的选择。实验表明，当选择Δλ = 100时，可同时对两种PAHs进行定性和定量测定。荧光强度与萘和菲的浓度在0.5 - 25.0 μg·L⁻¹范围内呈线性关系，相关系数分别为0.999 5和0.999 7。检测限均低于0.03 μg·L⁻¹，萘和菲的相对标准偏差分别为1.19%和1.80%（n = 7）。结果表明，该化合物可用同步荧光光谱法进行定性和定量分析。
