Mizoguchi T, Hirata K, Kobayashi S, Chikuma T
Department of Analytical Chemistry of Medicines, Showa Pharmaceutical University, Tokyo, Japan.
Pharmazie. 2012 Sep;67(9):765-7.
A high-performance liquid chromatographic assay for monitoring the antifungal drug fluconazole in human serum was developed using a C18 reversed-phase column with isocratic elution. The method involved sample clean-up by solid-phase column extraction, and subsequent analysis required only 14 min per sample for separation and quantitation. The assay was precise, with intra- and inter-assay relative standard deviations of < or = 1.5% and < or = 3.1%. The minimum detectable concentration of fluconazole was 0.3 nmol/ml. This assay has the advantage of minimizing the risk of interference from co-administered drugs to critically ill patients undergoing hemodiafiltration.
开发了一种用于监测人血清中抗真菌药物氟康唑的高效液相色谱分析法，该方法使用C18反相柱进行等度洗脱。该方法包括通过固相柱萃取进行样品净化，随后每个样品的分离和定量分析仅需14分钟。该分析方法精密度高，批内和批间相对标准偏差分别≤1.5%和≤3.1%。氟康唑的最低检测浓度为0.3 nmol/ml。该分析方法的优点是将同时使用的药物对接受血液透析滤过的重症患者的干扰风险降至最低。
