Marschner Clara B, Kristensen Annemarie T, Spodsberg Eva H, Wiinberg Bo
Department of Small Animal Clinical Sciences, Faculty of Life Sciences, University of Copenhagen, Denmark.
J Vet Emerg Crit Care (San Antonio). 2012 Feb;22(1):107-15. doi: 10.1111/j.1476-4431.2011.00709.x.
To investigate the performance of the Multiplate platelet function analyzer with regards to: (1) the use of 3 different anticoagulants (ie, citrate, hirudin, and heparin) and (2) the evaluation of optimal assay time.
Prospective observational in vitro study.
University veterinary teaching hospital.
Twenty clinically healthy dogs and 3 ill dogs.
None.
A total of 184 analyses were performed with duplicate measurements in each test cell and results are reported as mean of the 2 measurements. Analyses were performed on blood samples from 20 dogs collected in citrate, hirudin, or heparin. A total of 4 analyses were performed on every blood sample using adenosine diphosphate, collagen, and arachidonic acid as agonists as well as a control with 0.9% sodium chloride (buffer). Aggregation in hirudin samples was significantly increased compared with heparin at all analysis times except at 6 minutes when using ADP as agonist; however, hirudin samples also demonstrated significant aggregation in the buffer control, compared to both citrate and heparin. Citrated samples yielded significantly lower aggregation compared with both hirudin- and heparin-stabilized samples at 6 and 12 minutes when ADP and collagen were used as agonists, and at most analysis times with arachidonic acid. The assay performed best at shorter analyses times, whereas longer analyses times yielded larger variation in data.
There was a good aggregation response and acceptable analytical variation in both heparin- and hirudin-anticoagulated samples with all tested agonist at the concentrations recommended by the manufacturer. The results suggest that heparin may be superior as anticoagulant for Multiplate analyses in dogs and that short analyses times are preferable. Spontaneous platelet autoaggregation in hirudin samples warrants careful evaluation of results using this anticoagulant, especially at longer test times. The use of citrate is discouraged for Multiplate analyses in dogs due to a weak aggregation response.
研究多电极血小板功能分析仪在以下方面的性能：（1）使用3种不同的抗凝剂（即枸橼酸盐、水蛭素和肝素）；（2）评估最佳检测时间。
前瞻性体外观察研究。
大学兽医教学医院。
20只临床健康犬和3只患病犬。
无。
在每个测试单元中进行重复测量，共进行了184次分析，结果以两次测量的平均值报告。对采集于枸橼酸盐、水蛭素或肝素中的20只犬的血样进行分析。使用二磷酸腺苷、胶原和花生四烯酸作为激动剂以及0.9%氯化钠（缓冲液）作为对照，对每份血样进行4次分析。除使用ADP作为激动剂在6分钟时外，在所有分析时间，水蛭素样本中的聚集均显著高于肝素样本；然而，与枸橼酸盐和肝素样本相比，水蛭素样本在缓冲液对照中也显示出显著聚集。当使用ADP和胶原作为激动剂时，在6分钟和12分钟时，以及在使用花生四烯酸的大多数分析时间，枸橼酸盐样本的聚集显著低于水蛭素和肝素稳定的样本。该检测在较短的分析时间表现最佳，而较长的分析时间会导致数据变化更大。
在制造商推荐的浓度下，肝素和水蛭素抗凝的样本在所有测试激动剂下均有良好的聚集反应和可接受的分析变异。结果表明，肝素可能是犬多电极分析中更优的抗凝剂，且较短的分析时间更佳。水蛭素样本中的自发性血小板自聚集需要谨慎评估使用该抗凝剂的结果，尤其是在较长的检测时间。由于聚集反应较弱，不建议在犬的多电极分析中使用枸橼酸盐。
