Dugan Greg, O'Donnell Lisa, Hanbury David B, Cline J Mark, Caudell David L
a Department of Pathology , Center for Comparative Medicine Research, Wake Forest School of Medicine , Winston-Salem, NC , USA.
Platelets. 2015;26(8):730-5. doi: 10.3109/09537104.2014.988694. Epub 2014 Dec 30.
Electrical impedance aggregometry (EIA) has gained popularity in clinical and research applications. Nonhuman primates are used to study disease and drug-related mechanisms that affect hemostasis, therefore establishing normal EIA parameters are necessary. The anticoagulants sodium heparin, hirudin and sodium citrate and three agonists, ADP, ASPI, and collagen were evaluated. Whole blood from 12 adult male rhesus macaques was collected to evaluate anticoagulants, sodium heparin, hirudin and sodium citrate using three agonists (ADP, ASPI and collagen), on the Multiplate® 5.0 Analyzer. Platelet function was reported for three parameters: Area under the curve (AUC), aggregation, and aggregation velocity. There was a significant difference in mean AUC between citrate and heparin samples, and citrate and hirudin samples regardless of the agonist used. There was no difference in AUC between heparin and hirudin. ADP-activated samples showed an increase in impedance with hirudin samples compared to citrate. Furthermore, heparin and hirudin out-perform citrate as the anticoagulant for EIA in the macaque. Finally, this study demonstrates the utility of the Multiplate® system in this model and provides important insight into anticoagulant choice when using EIA.
电阻抗聚集法（EIA）在临床和研究应用中越来越受欢迎。非人灵长类动物被用于研究影响止血的疾病和药物相关机制，因此建立正常的EIA参数是必要的。评估了抗凝剂肝素钠、水蛭素和柠檬酸钠以及三种激动剂ADP、阿司匹林（ASPI）和胶原蛋白。采集12只成年雄性恒河猴的全血，在Multiplate® 5.0分析仪上使用三种激动剂（ADP、ASPI和胶原蛋白）评估抗凝剂肝素钠、水蛭素和柠檬酸钠。报告了血小板功能的三个参数：曲线下面积（AUC）、聚集率和聚集速度。无论使用何种激动剂，但柠檬酸盐与肝素样本之间以及柠檬酸盐与水蛭素样本之间的平均AUC存在显著差异。肝素和水蛭素之间的AUC没有差异。与柠檬酸盐相比，ADP激活的水蛭素样本的阻抗增加。此外，在猕猴中，肝素和水蛭素作为EIA的抗凝剂比柠檬酸盐表现更好。最后，本研究证明了Multiplate®系统在该模型中的实用性，并为使用EIA时抗凝剂的选择提供了重要见解。
