Zhang Yi, Madhu Vedavathi, Dighe Abhijit S, Irvine James N, Cui Quanjun
Department of Orthopaedic Surgery, University of Virginia, Charlottesville, VA 22908, USA.
Growth Factors. 2012 Oct;30(5):333-43. doi: 10.3109/08977194.2012.720574.
Exogenous addition of three factors-mesenchymal stem cells (MSCs), vascular endothelial growth factor (VEGF), and bone morphogenetic proteins (BMPs)-has proven to be more beneficial than delivery of any single factor for fracture repair in animal models. We studied the osteogenic differentiation of human adipose-derived stem cells (hADSCs) in the presence of VEGF, BMP-6, or VEGF plus BMP-6 to better understand their enhancement of osteoblastic differentiation of MSCs. The VEGF plus BMP-6 group demonstrated an additive effect on the enhancement of mineralization and expression of ALP and Msx2 genes. Unlike VEGF or BMP-6 alone, the combination of VEGF and BMP-6 significantly enhanced the expression of COL1A1, osterix, and Dlx5 genes. The data indicate that a cross-talk between VEGF and BMP-6 signaling pathways enhances osteogenic differentiation of hADSCs.
在动物模型中，外源性添加三种因子——间充质干细胞（MSC）、血管内皮生长因子（VEGF）和骨形态发生蛋白（BMP）——已被证明比单独递送任何一种因子对骨折修复更有益。我们研究了在VEGF、BMP-6或VEGF加BMP-6存在的情况下人脂肪来源干细胞（hADSC）的成骨分化，以更好地了解它们对MSC成骨细胞分化的增强作用。VEGF加BMP-6组在增强矿化以及碱性磷酸酶（ALP）和Msx2基因表达方面表现出相加效应。与单独使用VEGF或BMP-6不同，VEGF和BMP-6的组合显著增强了COL1A1、osterix和Dlx5基因的表达。数据表明，VEGF和BMP-6信号通路之间的相互作用增强了hADSC的成骨分化。
