骨形态发生蛋白-2、血管内皮生长因子和碱性成纤维细胞生长因子协同促进大鼠骨髓间充质干细胞的成骨分化。
BMP-2, VEGF and bFGF synergistically promote the osteogenic differentiation of rat bone marrow-derived mesenchymal stem cells.
机构信息
College of Materials Science and Engineering, Sichuan University, Chengdu, 610064, People's Republic of China.
出版信息
Biotechnol Lett. 2013 Mar;35(3):301-8. doi: 10.1007/s10529-012-1084-3. Epub 2012 Nov 10.
Mesenchymal stem cells (MSCs) were treated with bone morphogenetic protein-2 (BMP-2), vascular endothelial growth factor (VEGF) and basic fibroblast growth factor (bFGF) dose-dependently and time-dependently. Together they caused a strong synergistic effect on the osteogenic differentiation of MSCs, with lower concentrations of each factor being enough to show the synergistic promotion (50 ng BMP-2/ml, 1 ng VEGF/ml and 10 ng bFGF/ml). When both VEGF and bFGF were added in the early proliferating stage (the first 7 days) and BMP-2 was added in the late differentiation stage (the last 7 days), osteogenic differentiation of MSCs could be enhanced more effectively.
间充质干细胞（MSCs）经骨形成蛋白-2（BMP-2）、血管内皮生长因子（VEGF）和碱性成纤维细胞生长因子（bFGF）剂量依赖性和时间依赖性处理。它们共同对 MSCs 的成骨分化产生强烈的协同作用，每个因子的较低浓度足以显示协同促进作用（50ng/ml BMP-2、1ng/ml VEGF 和 10ng/ml bFGF）。当 VEGF 和 bFGF 在早期增殖阶段（第 1 天至第 7 天）添加，BMP-2 在后期分化阶段（第 7 天至第 14 天）添加时，MSCs 的成骨分化可以更有效地增强。
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