Institute of Cytology and Genetics, Siberian Branch of Russian Academy of Sciences, Novosibirsk 630090, Russia.
J Insect Physiol. 2012 Dec;58(12):1534-40. doi: 10.1016/j.jinsphys.2012.09.005. Epub 2012 Sep 24.
In Drosophila females, the precursor of 20-hydroxyecdysone (20E), ecdysone, is synthesized in the ovary follicular cells. Juvenile hormone (JH) is synthesized de novo in the specialized endocrine gland, corpus allatum (CA); JH degradation is carried out by the enzymes synthesized in the fat body (FB). Earlier we have shown that in Drosophila females, dopamine up- or down-regulates 20E and JH levels depending on the developmental stage. The present study focuses on the role of D1- and D2-like receptors in the age-specific regulation of 20E and JH metabolism by dopamine. We show that in Drosophila melanogaster females D1- and D2-like receptor genes (DopR and DD2R accordingly) are expressed in CA and FB, but not in follicular cells. The level of DopR expression in CA is much higher in the young than in the mature females, whereas DD2R expression is much higher in the mature than in the young females. The DopR and DD2R expression patterns in FB are opposite to those observed in CA. In addition, we obtained data suggesting that the age-specific changes of DopR and DD2R expression in FB are under the control of 20E.
在雌性果蝇中，20-羟基蜕皮激素（20E）的前体蜕皮激素在卵巢滤泡细胞中合成。保幼激素（JH）是在专门的内分泌腺-心侧体（CA）中从头合成的；JH 的降解是由脂肪体（FB）中合成的酶完成的。我们之前已经表明，在雌性果蝇中，多巴胺根据发育阶段上调或下调 20E 和 JH 水平。本研究重点关注 D1-和 D2-样受体在多巴胺对 20E 和 JH 代谢的年龄特异性调节中的作用。我们表明，在黑腹果蝇雌性中，D1-和 D2-样受体基因（相应的 DopR 和 DD2R）在 CA 和 FB 中表达，但不在滤泡细胞中表达。CA 中 DopR 的表达水平在年轻雌性中比成熟雌性高得多，而 DD2R 的表达水平在成熟雌性中比年轻雌性高得多。FB 中 DopR 和 DD2R 的表达模式与在 CA 中观察到的相反。此外，我们获得的数据表明，FB 中 DopR 和 DD2R 表达的年龄特异性变化受 20E 的控制。
