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脂肪分解活性与粘质沙雷氏菌的外膜共同纯化。

Lipolytic activity copurified with the outer membrane of Serratia marcescens.

作者信息

Heller K B

出版信息

J Bacteriol. 1979 Dec;140(3):1120-2. doi: 10.1128/jb.140.3.1120-1122.1979.

DOI:10.1128/jb.140.3.1120-1122.1979
PMID:230176
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC216763/
Abstract

Lipase, nuclease, and protease activities could be shown primarily with the purified outer membrane fraction from Serratia marcescens. These activities increased and decreased in the different compartments dependent on the growth phase of the cell culture. Penicillin-hydrolyzing activity was exclusively demonstrated with the outer membrane fraction.

摘要

脂肪酶、核酸酶和蛋白酶活性主要可通过粘质沙雷氏菌纯化的外膜组分来显示。这些活性在不同区室中随细胞培养物的生长阶段而增减。青霉素水解活性仅在外膜组分中得到证实。

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本文引用的文献

1
ISOLATION AND PROPERTIES OF AN EXOCELLULAR NUCLEASE OF SERRATIA MARCESCENS.粘质沙雷氏菌胞外核酸酶的分离与特性
J Bacteriol. 1963 Feb;85(2):273-8. doi: 10.1128/jb.85.2.273-278.1963.
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Micro-iodometric assay for penicillinase.青霉素酶的微量碘量法测定
Biochem J. 1962 May;83(2):236-40. doi: 10.1042/bj0830236.
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Isolation of covalently closed circular deoxyribonucleic acid from bacteria which produce exocellular nuclease.从产生胞外核酸酶的细菌中分离共价闭合环状脱氧核糖核酸。
J Bacteriol. 1973 Jan;113(1):508-9. doi: 10.1128/jb.113.1.508-509.1973.
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Mechanism of assembly of the outer membrane of Salmonella typhimurium. Isolation and characterization of cytoplasmic and outer membrane.鼠伤寒沙门氏菌外膜组装机制。细胞质膜和外膜的分离与特性分析。
J Biol Chem. 1972 Jun 25;247(12):3962-72.
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R factor-mediated antibiotic resistance in Serratia marcescens.粘质沙雷氏菌中R因子介导的抗生素耐药性。
Antimicrob Agents Chemother. 1976 Jul;10(1):123-7. doi: 10.1128/AAC.10.1.123.
6
Pullulanase, an enzyme of starch catabolism, is associated with the outer membrane of Klebsiella.支链淀粉酶是一种淀粉分解代谢酶，与克雷伯氏菌的外膜有关。
Arch Microbiol. 1978 Mar;116(3):303-10. doi: 10.1007/BF00417856.
7
Localization of proteolytic activity in the outer membrane of Escherichia coli.大肠杆菌外膜中蛋白水解活性的定位
J Bacteriol. 1979 Jan;137(1):574-83. doi: 10.1128/jb.137.1.574-583.1979.
8
The outer membrane proteins of Gram-negative bacteria: biosynthesis, assembly, and functions.革兰氏阴性菌的外膜蛋白：生物合成、组装及功能
Annu Rev Biochem. 1978;47:481-532. doi: 10.1146/annurev.bi.47.070178.002405.
9
Pleiotropic consequences of mutations towards antibiotic-hypersensitivity in Serratia marcescens.粘质沙雷氏菌中抗生素超敏性突变的多效性后果。
Arch Microbiol. 1978 Mar;116(3):259-68. doi: 10.1007/BF00417849.
10
Glycogen, hyaluronate, and some other polysaccharides greatly enhance the formation of exolipase by Serratia marcescens.糖原、透明质酸盐和其他一些多糖能极大地增强粘质沙雷氏菌外脂肪酶的形成。
J Bacteriol. 1979 Jun;138(3):663-70. doi: 10.1128/jb.138.3.663-670.1979.