Department of Pharmacology, Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Clinics (Sao Paulo). 2012 Sep;67(9):1077-85. doi: 10.6061/clinics/2012(09)16.
Osteoporosis increases the risk of bone fractures and may impair fracture healing. The aim of this study was to investigate whether alpha-tocopherol can improve the late-phase fracture healing of osteoporotic bones in ovariectomized rats.
In total, 24 female Sprague-Dawley rats were divided into three groups. The first group was sham-operated, and the other two groups were ovariectomized. After two months, the right femora of the rats were fractured under anesthesia and internally repaired with K-wires. The sham-operated and ovariectomized control rat groups were administered olive oil (a vehicle), whereas 60 mg/kg of alpha-tocopherol was administered via oral gavage to the alpha-tocopherol group for six days per week over the course of 8 weeks. The rats were sacrificed, and the femora were dissected out. Computed tomography scans and X-rays were performed to assess fracture healing and callus staging, followed by the assessment of callus strengths through the biomechanical testing of the bones.
Significantly higher callus volume and callus staging were observed in the ovariectomized control group compared with the sham-operated and alpha-tocopherol groups. The ovariectomized control group also had significantly lower fracture healing scores than the sham-operated group. There were no differences between the alpha-tocopherol and sham-operated groups with respect to the above parameters. The healed femora of the ovariectomized control group demonstrated significantly lower load and strain parameters than the healed femora of the sham-operated group. Alpha-tocopherol supplementation was not able to restore these biomechanical properties.
Alpha-tocopherol supplementation appeared to promote bone fracture healing in osteoporotic rats but failed to restore the strength of the fractured bone.
骨质疏松症会增加骨折的风险，并可能影响骨折愈合。本研究旨在探讨α-生育酚是否能改善去卵巢大鼠骨质疏松性骨折的晚期愈合。
共 24 只雌性 Sprague-Dawley 大鼠随机分为 3 组。第 1 组为假手术组，其余 2 组为去卵巢组。2 个月后，大鼠在麻醉下骨折，并用 K 线内固定修复。假手术和去卵巢对照组大鼠给予橄榄油（载体），而α-生育酚组给予 60mg/kgα-生育酚，每周 6 天，连续 8 周。处死大鼠，取出股骨。行 CT 扫描和 X 线检查评估骨折愈合和骨痂分期，然后通过骨生物力学试验评估骨痂强度。
与假手术组和α-生育酚组相比，去卵巢对照组的骨痂体积和骨痂分期明显更高。去卵巢对照组的骨折愈合评分也明显低于假手术组。α-生育酚组与假手术组在上述参数方面无差异。去卵巢对照组愈合的股骨的载荷和应变参数明显低于假手术组愈合的股骨。α-生育酚补充未能恢复这些生物力学特性。
α-生育酚补充似乎能促进骨质疏松大鼠的骨折愈合，但不能恢复骨折骨的强度。
