Department of Anatomy, Faculty of Medicine, University Kebangsaan Malaysia Medical Centre, Jalan Raja Muda Abdul Aziz, Kuala Lumpur, Malaysia.
Clinics (Sao Paulo). 2011;66(5):865-72. doi: 10.1590/s1807-59322011000500025.
Osteoporotic fractures are common during osteoporotic states. Piper sarmentosum extract is known to possess antioxidant and anti-inflammatory properties.
To observe the radiological changes in fracture calluses following administration of a Piper sarmentosum extract during an estrogen-deficient state.
A total of 24 female Sprague-Dawley rats (200-250 g) were randomly divided into 4 groups: (i) the sham-operated group; (ii) the ovariectomized-control group; (iii) the ovariectomized + estrogen-replacement therapy (ovariectomized-control + estrogen replacement therapy) group, which was supplemented with estrogen (100 μg/kg/day); and (iv) the ovariectomized + Piper sarmentosum (ovariectomized + Piper sarmentosum) group, which was supplemented with a water-based Piper sarmentosum extract (125 mg/kg). Six weeks after an ovariectomy, the right femora were fractured at the mid-diaphysis, and a K-wire was inserted. Each group of rats received their respective treatment for 6 weeks. Following sacrifice, the right femora were subjected to radiological assessment.
The mean axial callus volume was significantly higher in the ovariectomized-control group (68.2 ± 11.74 mm³) than in the sham-operated, estrogen-replacement-therapy and Piper sarmentosum groups (20.4 ± 4.05, 22.4 ± 4.14 and 17.5 ± 3.68 mm³, respectively). The median callus scores for the sham-operated, estrogen-replacement-therapy and Piper sarmentosum groups had median (range, minimum - maximum value) as 1.0 (0 - 2), 1.0 (1 - 2) and 1.0 (1 - 2), respectively, which were significantly lower than the ovariectomized-control group score of 2.0 (2 - 3). The median fracture scores for the sham-operated, estrogen-replacement-therapy and Piper sarmentosum groups were 3.0 (3 - 4), 3.0 (2 - 3) and 3.0 (2 - 3), respectively, which were significantly higher than the ovariectomized-control group score of 2.0 (1 - 2) (p<0.05).
The Piper sarmentosum extract improved fracture healing, as assessed by the reduced callus volumes and reduced callus scores. This extract is beneficial for fractures in osteoporotic states.
骨质疏松症状态下常发生骨质疏松性骨折。众所周知，Piper sarmentosum 提取物具有抗氧化和抗炎特性。
观察在雌激素缺乏状态下给予 Piper sarmentosum 提取物后骨折愈合过程中骨痂的放射学变化。
将 24 只雌性 Sprague-Dawley 大鼠（200-250g）随机分为 4 组：（i）假手术组；（ii）卵巢切除对照组；（iii）卵巢切除+雌激素替代治疗组（卵巢切除+雌激素替代治疗），补充雌激素（100μg/kg/天）；（iv）卵巢切除+Piper sarmentosum 组（卵巢切除+Piper sarmentosum），补充水基 Piper sarmentosum 提取物（125mg/kg）。卵巢切除 6 周后，将右侧股骨中段骨折，插入 K 线。每组大鼠接受相应的治疗 6 周。处死大鼠后，对右侧股骨进行放射学评估。
卵巢切除对照组的平均轴向骨痂体积（68.2±11.74mm³）明显高于假手术组、雌激素替代治疗组和 Piper sarmentosum 组（20.4±4.05、22.4±4.14 和 17.5±3.68mm³）。假手术组、雌激素替代治疗组和 Piper sarmentosum 组的骨痂评分中位数（范围，最小值-最大值）分别为 1.0（0-2）、1.0（1-2）和 1.0（1-2），均明显低于卵巢切除对照组的 2.0（2-3）。假手术组、雌激素替代治疗组和 Piper sarmentosum 组的骨折评分中位数分别为 3.0（3-4）、3.0（2-3）和 3.0（2-3），均明显高于卵巢切除对照组的 2.0（1-2）（p<0.05）。
Piper sarmentosum 提取物通过减少骨痂体积和降低骨痂评分来改善骨折愈合。该提取物对骨质疏松性骨折有益。
