Bertram E H, Lothman E W, Lenn N J
Department of Neurology, University of Virginia Health Sciences Center, Charlottesville 22908.
Ann Neurol. 1990 Jan;27(1):43-8. doi: 10.1002/ana.410270108.
The effect of intermittent seizures on the pyramidal neurons of the hippocampus is largely unknown. To determine whether recurrent seizures centered in the hippocampus can produce neuronal loss in this region, a morphometric analysis was performed from standardized sections of hippocampus using 5 groups of animals: (1) surgical control subjects, (2) rats kindled by the rapidly recurring hippocampal seizure (RRHS) paradigm, (3) kindled rats with a few additional limbic seizures (528 +/- 66 seizures), (4) kindled rats with many limbic seizures (1,523 +/- 130 seizures), and (5) rats experiencing limbic status epilepticus (SE) induced by "continuous" hippocampal stimulation. The RRHS and SE protocols induced significant neuronal loss in the CA1 region, but no evidence was found for additional cell loss with increasing numbers of intermittent seizures. These intermittent seizures were, however, associated with a significant thickening of the basal and apical dendritic fields of the CA1 region. These findings indicate that intermittent seizures produce no significant hippocampal neuronal loss and may result in a hypertrophy of CA1 dendritic fields.
间歇性癫痫发作对海马体锥体细胞的影响在很大程度上尚不明确。为了确定以海马体为中心的反复癫痫发作是否会导致该区域神经元丧失，我们使用5组动物，对海马体的标准化切片进行了形态计量分析：（1）手术对照组；（2）通过快速反复海马体癫痫发作（RRHS）模式点燃的大鼠；（3）伴有少量额外边缘系统癫痫发作（528±66次发作）的点燃大鼠；（4）伴有多次边缘系统癫痫发作（1523±130次发作）的点燃大鼠；（5）通过“持续”海马体刺激诱发边缘系统癫痫持续状态（SE）的大鼠。RRHS和SE方案均导致CA1区出现显著的神经元丧失，但未发现随着间歇性癫痫发作次数增加而出现额外细胞丧失的证据。然而，这些间歇性癫痫发作与CA1区基底和顶端树突野的显著增粗有关。这些发现表明，间歇性癫痫发作不会导致显著的海马体神经元丧失，可能会导致CA1树突野肥大。
