Laboratory of Molecular Immunology, The Rockefeller University, New York, NY 10065, USA.
Proc Natl Acad Sci U S A. 2012 Sep 25;109(39):15859-64. doi: 10.1073/pnas.1213409109. Epub 2012 Sep 10.
Passive transfer of neutralizing antibodies against HIV-1 can prevent infection in macaques and seems to delay HIV-1 rebound in humans. Anti-HIV antibodies are therefore of great interest for vaccine design. However, the basis for their in vivo activity has been difficult to evaluate systematically because of a paucity of small animal models for HIV infection. Here we report a genetically humanized mouse model that incorporates a luciferase reporter for rapid quantitation of HIV entry. An antibody's ability to block viral entry in this in vivo model is a function of its bioavailability, direct neutralizing activity, and effector functions.
被动转移针对 HIV-1 的中和抗体可以预防猕猴感染，并且似乎可以延缓人类 HIV-1 的反弹。因此，抗 HIV 抗体对于疫苗设计具有重要意义。然而，由于缺乏用于 HIV 感染的小型动物模型，其体内活性的基础一直难以系统地评估。在这里，我们报告了一种基因人源化的小鼠模型，该模型包含一个荧光素酶报告基因，可快速定量 HIV 进入。抗体在该体内模型中阻断病毒进入的能力是其生物利用度、直接中和活性和效应功能的函数。
