Zhou Tao, Zhang Xiaobo, Guo Lanping, Lin Ge, Jiang Weike, Ai Qiang, Zhang Chenggang
Guiyang College of Traditional Chinese Medicine, Guiyang 550002, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jul;37(13):1917-21.
Through comparative study on contents of icariin and total flavonoid of Epimedium acuminatum in different habitats and parts, the distribution and correlation of the two components were observed.
Twenty-four sample spots in four habitats were set up, and the whole plant of these samples was divided as following: leaf, root, stem and rhizome. Total flavonoid and icariin of samples were determined by using the UV method and the HPLC, respectively. The data was analyzed by SPSS 17.0.
The distribution of icariin in different parts had the pattern: leaf > root > stem > rhizome, the total flavonoid content was higher in leaf, but it showed no difference in other parts. Habitat had certain effect on icariin accumulation in E. acuminatum. The significant differences of total flavonoid content in E. acuminatum from different parts were not observed.
The distribution of icariin from different parts and habitats has high selectivity. Metabolism and accumulation of flavonoid content in each part have no difference. Flavonoid content is less affected by environment. Considering the distribution of icariin and flavonoid content in every part and the growth strategy in different habitats comprehensively, it is reasonable to harvest the aerial part for the sustainable utilization of E. acuminatum.
通过对不同产地及部位粗毛淫羊藿中淫羊藿苷和总黄酮含量的比较研究，观察二者的分布及相关性。
在4个产地设置24个采样点，将采集样本的全株分为叶、根、茎、根茎。分别采用紫外分光光度法和高效液相色谱法测定样本中总黄酮和淫羊藿苷的含量。数据采用SPSS 17.0软件进行分析。
淫羊藿苷在不同部位的分布规律为：叶＞根＞茎＞根茎；总黄酮含量在叶中较高，其他部位差异不显著。产地对粗毛淫羊藿中淫羊藿苷的积累有一定影响。不同部位粗毛淫羊藿总黄酮含量未观察到显著差异。
淫羊藿苷在不同部位和产地的分布具有较高选择性。各部位黄酮类成分的代谢和积累无差异。黄酮类成分受环境影响较小。综合考虑淫羊藿苷和黄酮类成分在各部位的分布以及不同产地的生长策略，采收地上部分用于粗毛淫羊藿的可持续利用较为合理。
