Li Xiao-Ming, Pan Jun-Qian, Luo Yan-Jiao, Yang Qian-Ru, Xu Chao-Qun, Shen Guo-An, Wei Sheng-Hua, Guo Bao-Lin
School of Pharmacy, Guizhou University of Traditional Chinese Medicine Guiyang 550025, China Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100193, China.
Institute of Medicinal Plant Development, Chinese Academy of Medical Sciences, Peking Union Medical College Beijing 100193, China Institute of Radiation Medicine, Academy of Military Medical Sciences Beijing 100850, China.
Zhongguo Zhong Yao Za Zhi. 2020 Jun;45(11):2502-2508. doi: 10.19540/j.cnki.cjcmm.20200329.113.
In this study, the growth index including plant height, compound leaf area, specific leaf area, leaf water content, number of branches, and leaf biomass per plant and the icariin flavonoids such as epimedin A, epimedin B, epimedin C and icariin of Epimedium pseudowushanense were determined on 30 d and 60 d under light intensity(18.2±2.5) μmol·m(-2)·s(-1)(L1) and(90.9 ±2.5) μmol·m(-2)·s(-1)(L2), and white light as control, red light, blue light and yellow light were used as three light quality treatments, to study the effect of light quality on the growth and flavonoids accumulation of E. pseudowushanense. The E. pseudowushanense was sui-table for growth under L1 light intensity, the blue light treatment significantly reduced the leaf area, but had little effect on the stem height, the red light treatment and the yellow light treatment had no obvious effect on the stem height and leaf area, but the yellow light treatment significantly increased the germination of new branches, and had a sustained promoting effect, and the biomass was significantly higher than the white light treatment at 60 d. The content of icariin flavonoids in red light, blue light and yellow light treatment was higher than that in white light treatment at 30 d and 60 d under L1 light intensity, while yellow light treatment promoted the synthesis of icariin flavonoids to the largest extent, which was 1.8 and 1.9 times of white light treatment(30 d and 60 d).Under L2 light intensity, the effect of strong light on promoting stem germination became the main factor, while the yellow light treatment showed no significant effect on promoting stem germination, and the red light treatment exhibited a significant effect on reducing leaf area. Icariin flavonoids under red light, blue light and yellow light treatment were all lower than that under white light treatment, that is, the effect of white light treatment on the synthesis of icariin flavonoids is better than red light, blue light and yellow light treatment. When the time of strong light treatment was longer, the degradation range of icariin flavonoids in other light treatment appeared, while red light treatment promotes the synthesis of icariin flavonoids. Therefore, the influence of light quality on E. pseudowushanense is quite different under different light intensity, no matter from growth index or flavonoid content index. The results support that the biomass and icariin flavonoid content can be increased by providing appropriate red and yellow light.
本研究在光强为(18.2±2.5)μmol·m⁻²·s⁻¹(L1)和(90.9±2.5)μmol·m⁻²·s⁻¹(L2)条件下，于30 d和60 d测定了巫山淫羊藿的生长指标，包括株高、复叶面积、比叶面积、叶片含水量、分枝数和单株叶片生物量，以及淫羊藿苷类黄酮如朝藿定A、朝藿定B、朝藿定C和淫羊藿苷的含量，以白光为对照，采用红光、蓝光和黄光作为三种光质处理，研究光质对巫山淫羊藿生长及黄酮类化合物积累的影响。巫山淫羊藿在L1光强下适合生长，蓝光处理显著降低了叶面积，但对茎高影响较小，红光处理和黄光处理对茎高和叶面积无明显影响，但黄光处理显著增加了新枝萌发，并具有持续促进作用，60 d时生物量显著高于白光处理。在L1光强下，30 d和60 d时红光、蓝光和黄光处理中淫羊藿苷类黄酮含量均高于白光处理，而黄光处理对淫羊藿苷类黄酮合成的促进作用最大，分别是白光处理的1.8倍和1.9倍(30 d和60 d)。在L2光强下，强光促进茎萌发的作用成为主要因素，而黄光处理对促进茎萌发无显著影响，红光处理对减小叶面积有显著影响。红光、蓝光和黄光处理下的淫羊藿苷类黄酮均低于白光处理，即白光处理对淫羊藿苷类黄酮合成的效果优于红光、蓝光和黄光处理。当强光处理时间较长时，其他光处理下淫羊藿苷类黄酮出现降解，而红光处理促进淫羊藿苷类黄酮的合成。因此，无论从生长指标还是黄酮含量指标来看，不同光强下光质对巫山淫羊藿的影响差异较大。研究结果支持通过提供适宜的红光和黄光可提高生物量和淫羊藿苷类黄酮含量。
