Yang Xuedong, Wu Xun, Hu Licui, Guo Henan
School of Pharmaceutical Science and Technology, Tianjin University, Tianjin 300072, China.
Zhongguo Zhong Yao Za Zhi. 2012 Jul;37(13):1942-5.
To compare the effect of different solvents such as water, ethanol, methanol, ethyl acetate and petroleum ether on extraction of 10 effective components from Ligusticum chuanxiong and component characteristics of corresponding extracts.
Ultrasonic assisted solvent extraction and high performance liquid chromatography quantitative analysis were adopted to determine effective components. CAPCELL PAK C18 column (4.6 mm x 150 mm, 5 microm) was adopted. The mobile phase was methanol-0. 5% HAc for gradient elute. The detection wavelength was 280 nm. The column temperature was 30 degrees C. The flow rate was 0. 7 mL x min(-1). The sample size was 10 microL.
Methanol or ethanol showed no significant difference in extraction of ferulic acid, hydroxyphthalide, alkylphthalide and diligustilide. Ethyl acetate displayed relatively low extraction ratios in hydroxyphthalide and ferulic acid. Water and petroleum ether showed relatively low extraction ratios in all four effective components, and water extracts showed different component characteristics.
Ethanol and methanol are the most suitable solvents to extract four effective components from L. chuanxiong.
比较水、乙醇、甲醇、乙酸乙酯和石油醚等不同溶剂对川芎中10种有效成分的提取效果及相应提取物的成分特征。
采用超声辅助溶剂萃取法和高效液相色谱定量分析法测定有效成分。采用CAPCELL PAK C18柱（4.6 mm×150 mm，5μm）。流动相为甲醇 - 0.5% HAc进行梯度洗脱。检测波长为280 nm。柱温为30℃。流速为0.7 mL·min⁻¹。进样量为10μL。
甲醇或乙醇在阿魏酸、羟基苯酞、烷基苯酞和二氢藁本内酯的提取上无显著差异。乙酸乙酯在羟基苯酞和阿魏酸中的提取率相对较低。水和石油醚在所有四种有效成分中的提取率相对较低，且水提取物显示出不同的成分特征。
乙醇和甲醇是从川芎中提取四种有效成分的最合适溶剂。
