[全蝎与蜈蚣对胶原诱导性关节炎（CIA）大鼠外周血CD4 + CD25 + FoxP3 +调节性T细胞的调控作用]
[Regulation effect of scorpio and scolopendra on CD4 + CD25 + FoxP3 + Treg cell in peripheral blood from rats with collagen-induced arthritis (CIA)].
作者信息
Liu Duan-Yong, Zhao Hai-Mei, Huang Xiao-Ying, Zuo Zhi-Qin, Wang Yan-Hui, Cheng Shao-Min
机构信息
Technology College, Jiangxi University of Traditional Chinese Medicine Science, Nanchang 330025, China.
出版信息
Zhong Yao Cai. 2012 Apr;35(4):525-8.
OBJECTIVE
To study the regulation effect of Scorpio and Scolopendra on CD4 + CD25 + FoxP3 + Treg Cell in peripheral blood from rats with collagen-induced arthritis.
METHODS
Sixty female Wistar rats were randomly divided into 6 groups, normal control group, model control group, low-dose, middle-dose and high-dose Scorpio and Scolopendra group,and the type II collagen group. Rats' arthritis was induced by collagen. The number of CD4 + CD25 + FoxP3 + Treg cell in peripheral blood was tested by flow cytometry, and the level of IL-2 in serum was detected by enzyme linked immunosorbent assay.
RESULTS
Compared with the model groups,the levels of CD4 + CD25 + Treg cell and CD4 + CD25 + FoxP3 + Treg cell were increased obviously in the high and low dose of Scorpio and Scolopendra groups (P < 0.05 or P < 0.01), while the level of IL-2 in serum was decreased remarkably in the middle and low dose of Scorpio and Scolopendra groups (P < 0.05 or P < 0.01).
CONCLUSION
It is realized that Scorpio and Scolopendra effectively treat RA by regulating the level of CD4 + CD25 + FoxP3 + Treg cell to restore immunological tolerance.
目的
研究全蝎与蜈蚣对胶原诱导性关节炎大鼠外周血CD4+CD25+FoxP3+调节性T细胞的调控作用。
方法
将60只雌性Wistar大鼠随机分为6组，即正常对照组、模型对照组、低剂量全蝎与蜈蚣组、中剂量全蝎与蜈蚣组、高剂量全蝎与蜈蚣组以及Ⅱ型胶原组。采用胶原诱导大鼠发生关节炎。通过流式细胞术检测外周血中CD4+CD25+FoxP3+调节性T细胞数量，采用酶联免疫吸附测定法检测血清中白细胞介素-2水平。
结果
与模型组相比，高剂量和低剂量全蝎与蜈蚣组CD4+CD25+调节性T细胞及CD4+CD25+FoxP3+调节性T细胞水平明显升高（P<0.05或P<0.01），而中剂量和低剂量全蝎与蜈蚣组血清中白细胞介素-2水平显著降低（P<0.05或P<0.01）。
结论
认识到全蝎与蜈蚣可通过调节CD4+CD25+FoxP3+调节性T细胞水平来恢复免疫耐受，从而有效治疗类风湿关节炎。
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