Zhang Yun, Wang Jie, Guo Li-Li
Guanganmen Hospital, China Academy of Chinese Medical Sciences, Beijing.
Zhongguo Zhong Xi Yi Jie He Za Zhi. 2012 Jul;32(7):939-43.
To study the protection effects and the mechanisms of Huoxue Anxin Recipe (HAR) on acute myocardial infarction (AMI) rats.
The AMI Wistar rat model was prepared by ligating the left anterior descending coronary artery. By taking elantan long as the positive control drug, HAR was extracted from Chinese herbs by modern pharmacological methods and composed according to theories of Chinese medicine (CM). The medication time started from the day of modeling to the 21st day of the modeling. The heart function, the morphological changes of the heart, changes in the mRNA and protein levels of toll like receptor 4 nuclear factor kappa B tumor necrosis factor alpha (TLR4-NFkappaB-TNFalpha) pathway were observed.
Compared with the sham-operation group, the ejection fraction (EF) and left ventricular fractional shortening (FS) significantly decreased (P < 0.01), the left ventricular intemal dimension end-diastolic (LVIDd) and left ventricular internal dimension end-systolic (LVIDs) significantly increased (P < 0.01), the mRNA and protein levels of TLR4-NFkappaB-TNFalpha channel significantly increased in the model group (P < 0.01). The infarction in the front wall of the left ventricle was obviously seen, accompanied with severe inflammatory cell infiltration and collagen deposition in model group. Compared with the model group, the EF and FS significantly increased, LVIDd and LVIDs significantly decreased in the positive control group, the high and low dose HAR groups (P < 0.05, P < 0.01). The infracted area of the front wall of the left ventricle was obviously contracted. The inflammatory cell infiltration and collagen deposition were obviously alleviated. In the high and low dose HAR groups, the mRNA and protein expression levels of TLR4-NFkappaB-TNFalpha significantly decreased (P < 0.05, P < 0.01), but no inhibition was found in the positive control group.
HAR could significantly improve the morphological structures and functional abnormality induced by myocardial ischemia in AMI rats. Its effects was correlated with inhibiting TLR4-NFkappaB-TNFalpha pathway.
研究活血安心方（HAR）对急性心肌梗死（AMI）大鼠的保护作用及其机制。
通过结扎左冠状动脉前降支制备AMI Wistar大鼠模型。以依那普利作为阳性对照药物，采用现代药理方法从中药中提取HAR，并依据中医理论组方。给药时间从造模当天开始至造模后第21天。观察心功能、心脏形态学变化、Toll样受体4-核因子κB-肿瘤坏死因子α（TLR4-NFκB-TNFα）通路mRNA和蛋白水平的变化。
与假手术组相比，模型组射血分数（EF）和左心室短轴缩短率（FS）显著降低（P<0.01），左心室舒张末期内径（LVIDd）和左心室收缩末期内径（LVIDs）显著增加（P<0.01），模型组TLR4-NFκB-TNFα通道mRNA和蛋白水平显著升高（P<0.01）。模型组可见左心室前壁梗死明显，伴有严重的炎性细胞浸润和胶原沉积。与模型组相比，阳性对照组、HAR高剂量组和低剂量组EF和FS显著升高，LVIDd和LVIDs显著降低（P<0.05，P<0.01）。左心室前壁梗死面积明显缩小。炎性细胞浸润和胶原沉积明显减轻。HAR高剂量组和低剂量组TLR4-NFκB-TNFα的mRNA和蛋白表达水平显著降低（P<0.05，P<0.01），但阳性对照组未见抑制作用。
HAR可显著改善AMI大鼠心肌缺血所致的形态结构和功能异常。其作用与抑制TLR4-NFκB-TNFα通路有关。
